Syringolin A, a new plant elicitor from the phytopathogenic bacterium Pseudomonas syringae pv. syringae, inhibits the proliferation of neuroblastoma and ovarian cancer cells and induces apoptosis.

Abstract

Syringolin A is a new plant elicitor produced by the plant pathogen Pseudomonas syringae pv. syringae. The goal of this study was to investigate whether syringolin A exhibits anti-proliferative properties in cancer cells. The treatment of human neuroblastoma (NB) cells (SK-N-SH and LAN-1) and human ovarian cancer cells (SKOV3) with syringolin A (0-100 microm) inhibited cell proliferation in a dose-dependent manner. The IC(50) (50% inhibition) for each cell line ranged between 20 microm and 25 microm. In SK-N-SH cells, the treatment with 20 microm syringolin A led to a rapid (24 h) increase of the apoptosis-associated tumour suppressor protein p53. In addition, we found that the treatment of SK-N-SH cells caused severe morphological changes after 48 h such as rounding of cells and loss of adherence, both conditions observed during apoptosis. The induction of apoptosis by syringolin A was confirmed by both poly (ADP-ribose) polymerase (PARP) cleavage and annexin V assay. Taken together, we show for the first time that the natural product syringolin A exhibits anti-proliferative activity and induces apoptosis. Syringolin A and structurally modified syringolin A derivatives may serve as new lead compounds for the development of novel anticancer drugs.

• Authors: C S Coleman, J P Rocetes, D J Park, C J Wallick, B J Warn-Cramer, K Michel, R Dudler, A S Bachmann
• Journal: Cell proliferation (Cell Prolif) Vol. 39 Issue 6 Pg. 599-609 (Dec 2006) ISSN: 0960-7722 [Print] England
• PMID: 17109642 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Peptides, Cyclic
• Tumor Suppressor Protein p53
• syringolin A
• Poly(ADP-ribose) Polymerases
• Proto-Oncogene Proteins c-akt
• Caspase 3

Topics

• Animals
• Antineoplastic Agents (chemistry, pharmacology)
• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Cell Division (drug effects)
• Cell Line, Transformed
• Cell Line, Tumor
• Dose-Response Relationship, Drug
• Female
• Fibroblasts (cytology)
• Humans
• Nervous System Neoplasms (pathology)
• Neuroblastoma (pathology)
• Ovarian Neoplasms (pathology)
• Peptides, Cyclic (chemistry, pharmacology)
• Poly(ADP-ribose) Polymerases (metabolism)
• Proto-Oncogene Proteins c-akt (metabolism)
• Pseudomonas syringae (chemistry)
• Rats
• Tumor Suppressor Protein p53 (metabolism)