C/EBPbeta is over-expressed in gastric carcinogenesis and is associated with COX-2 expression.

Abstract

The CCAAT/enhancer-binding protein beta (C/EBPbeta) transcription factor has been associated with several cancer models. In this study, the expression of C/EBPbeta was analysed in a series of 90 gastric carcinomas (GCs). We also assessed the effect of C/EBPbeta on COX-2 expression. In normal gastric mucosa, C/EBPbeta expression was restricted to cells in the proliferative zone. In intestinal metaplasia, dysplasia, and GC of the intestinal and atypical subtypes, C/EBPbeta was over-expressed (p < 0.0001, for the association with histological type). C/EBPbeta and Ki67, a marker of cell proliferation, were also co-expressed in primary GC. We also observed an overlap between C/EBPbeta and COX-2 expression in GC. Using GC cell lines we show that C/EBPbeta can regulate the expression of endogenous COX-2 and transactivate the promoter of the COX-2 gene, depending on its methylation status. These results suggest that C/EBPbeta may be a marker of neoplastic transformation and also play an active role in gastric tumourigenesis by regulating COX-2 expression.

• Authors: G Regalo, P Canedo, G Suriano, C Resende, M L Campos, M J Oliveira, C Figueiredo, P Rodrigues-Pereira, N Blin, R Seruca, F Carneiro, J C Machado
• Journal: The Journal of pathology (J Pathol) Vol. 210 Issue 4 Pg. 398-404 (Dec 2006) ISSN: 0022-3417 [Print] England
• PMID: 16981245 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• CCAAT-Enhancer-Binding Protein-beta
• Ki-67 Antigen
• Cyclooxygenase 2

Topics

• Adult
• Aged
• Biomarkers, Tumor (analysis)
• CCAAT-Enhancer-Binding Protein-beta (genetics)
• Cell Division (physiology)
• Cell Line, Tumor
• Cell Transformation, Neoplastic (genetics, pathology)
• Cyclooxygenase 2 (genetics)
• Female
• Gastric Mucosa (pathology)
• Gene Expression Regulation, Neoplastic (genetics)
• Humans
• Immunohistochemistry (methods)
• Ki-67 Antigen (analysis)
• Male
• Metaplasia (genetics, pathology)
• Middle Aged
• Neoplasm Invasiveness
• Stomach Neoplasms (genetics, pathology, secondary)
• Tumor Cells, Cultured