Auto-antibodies in prostate cancer: humoral immune response to antigenic determinants coded by the differentially expressed transcripts FLJ23438 and VAMP3.

Abstract

BACKGROUND:

Here we evaluate auto-antibody response against two potential antigenic determinants of genes highly expressed in low Gleason Score prostate cancer (PC) tumor samples, namely FLJ23438 and VAMP3.

METHODS:

RT-PCR assays were used to analyze mRNA expression profiles of FLJ23438 and VAMP3 transcripts. The auto-antibody response against FLJ23438 and VAMP3 recombinant proteins was tested by immunoblot assays using PC, benign prostate hyperplasia (BPH), healthy donors (HD), and other human cancers plasma samples.

RESULTS:

Our data showed that 37% (10/27) and 7.4% (2/27) of PC plasma samples presented auto-antibodies against FLJ23438 and VAMP3, respectively. Only 8.3% (1/12) of BPH plasma samples were reactive for both auto-antibodies, while none (0/12) of HD plasma samples tested were reactive.

CONCLUSIONS:

The prevalence of 37% of positive PC plasma samples for anti-FLJ23438 antibodies suggests that humoral immune response against this antigenic determinant could be a potential serum marker for this cancer.

• Authors: E R Pontes, L C Matos, E A da Silva, L S Xavier, B L Diaz, I A Small, E M Reis, S Verjovski-Almeida, M A Barcinski, E R P Gimba
• Journal: The Prostate (Prostate) Vol. 66 Issue 14 Pg. 1463-73 (Oct 01 2006) ISSN: 0270-4137 [Print] United States
• PMID: 16897729 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: (c) 2006 Wiley-Liss, Inc.

Chemical References

• Antigens
• Autoantibodies
• Biomarkers, Tumor
• RNA, Messenger
• RNA, Small Nuclear
• Recombinant Proteins
• U2 small nuclear RNA
• VAMP3 protein, human
• Vesicle-Associated Membrane Protein 3

Topics

• Adenocarcinoma (epidemiology, immunology, physiopathology)
• Aged
• Antigens (genetics, immunology)
• Autoantibodies (blood)
• Biomarkers, Tumor (blood, genetics, immunology)
• Breast Neoplasms
• Carcinoma, Squamous Cell
• Cell Line, Tumor
• Colorectal Neoplasms
• Esophageal Neoplasms
• Gene Expression Regulation, Neoplastic
• Humans
• Lung Neoplasms
• Male
• Middle Aged
• Open Reading Frames (genetics)
• Prostatic Hyperplasia (epidemiology, immunology, physiopathology)
• Prostatic Neoplasms (epidemiology, immunology, physiopathology)
• RNA, Messenger (genetics)
• RNA, Small Nuclear (genetics, immunology)
• Recombinant Proteins (genetics, immunology)
• Seroepidemiologic Studies
• Vesicle-Associated Membrane Protein 3 (genetics, immunology)