[The expression and clinical significance of hematopoietic cell phosphatase and cysteinyl aspartate-specific proteinase in leukemia].

Abstract

OBJECTIVE:

To evaluate the relationship between the expression of hematopoietic cell phosphatase (SHP-1) gene or cysteinyl aspartate-specific proteinases (Caspase-1, 3) gene and the effect of chemotherapy in leukemia patients.

METHODS:

The expression of SHP-1 and Caspase-1, 3 gene was measured in leukemia patients with reverse transcriptase-polymerase chain reaction.

RESULTS:

The positive rate of SHP-1 and Caspase-1, 3 gene in acute leukemia was 33.3%, 80.0% and 100.0%. The positive rate of SHP-1 in chronic myeloid leukemia (CML)-blastic phase and CML-chronic phase was 0.0%, 37.5%. The first complete remission (CR) rate of SHP-1 and Caspase-1, 3 positive group was 85.0%, 64.3% and 55.8%, which were higher than that of SHP-1 and Caspase-1, 3 negative group (P < 0.05). There was a positive correlation between SHP-1 and Caspase-1, 3.

CONCLUSIONS:

SHP-1 gene is potentially an anti-oncogene and may play a role through activation of Caspase-1, 3 gene. The expression of SHP-1 and Caspase-1, 3 is associated with remission rate and it is shown that they participate in the prognosis of leukemia. They could be used as indices for evaluating therapeutic efficacy and judging prognosis in leukemia patients.

• Authors: Ying Han, Jian-min Luo, Xiao-hui Jia, Fu-xu Wang, Li Yao, Xing-yan Du
• Journal: Zhonghua nei ke za zhi (Zhonghua Nei Ke Za Zhi) Vol. 45 Issue 5 Pg. 363-5 (May 2006) ISSN: 0578-1426 [Print] China
• PMID: 16780734 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Messenger
• Protein Tyrosine Phosphatase, Non-Receptor Type 6
• Caspase 3
• Caspase 1

Topics

• Adolescent
• Adult
• Aged
• Apoptosis
• Bone Marrow Cells (metabolism)
• Caspase 1 (biosynthesis, genetics)
• Caspase 3 (biosynthesis, genetics)
• Female
• Gene Expression
• Humans
• Leukemia (metabolism, pathology)
• Male
• Middle Aged
• Protein Tyrosine Phosphatase, Non-Receptor Type 6 (biosynthesis, genetics)
• RNA, Messenger (genetics)
• Reverse Transcriptase Polymerase Chain Reaction