TATA-binding protein and TBP-associated factors during herpes simplex virus type 1 infection: localization at viral DNA replication sites.

Abstract

Host RNA polymerase II (RNAP II) is responsible for viral transcription of the herpes simplex virus type 1 (HSV-1) genome and is relocalized to viral DNA replication compartments. Thus, we investigated whether TATA-binding protein (TBP) and TBP-associated factors (TAFs) are recruited to sites of viral transcription and replication and whether TBP/TAF expressions are influenced upon infection. The protein levels of TBP, hsTAF1/TAF(II)250, hsTAF4/TAF(II)135, and hsTAF5/TAF(II)100 were constant during the early phase of infection and started to decrease late during infection. Only for hsTAF7/TAF(II)55 we sometimes observed a decrease already at 4-8h postinfection (p.i.). Concomitantly with the relocalization of RNAP II, TBP and hsTAFs were redistributed to sites of viral DNA replication and transcription. In the absence of viral DNA replication TBP/hsTAFs were present in distinct nuclear dots, however, enlargement of the nuclear structures did not take place. Our results show that HSV-1 infection has no influence on the protein levels of TFIID components and leads to a redistribution of TBP and hsTAFs to prereplicative sites that enlarge to viral DNA replication compartments.

• Authors: Ilja Quadt, Andrea Katharina Günther, Daniel Voss, Mario Schelhaas, Dagmar Knebel-Mörsdorf
• Journal: Virus research (Virus Res) Vol. 115 Issue 2 Pg. 207-13 (Feb 2006) ISSN: 0168-1702 [Print] Netherlands
• PMID: 16271277 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Viral
• TATA-Binding Protein Associated Factors
• TATA-Box Binding Protein
• RNA Polymerase II

Topics

• Cell Nucleus (chemistry)
• Cells, Cultured
• DNA Replication
• DNA, Viral (metabolism)
• Fibroblasts
• Herpesvirus 1, Human (physiology)
• Humans
• Immunohistochemistry
• Microscopy, Confocal
• RNA Polymerase II (analysis)
• TATA-Binding Protein Associated Factors (analysis)
• TATA-Box Binding Protein (analysis)
• Transcription, Genetic
• Virus Replication