[Compound nylestriol tablet to prevent bone loss in osteoporotic rats].

Abstract

OBJECTIVE:

To investigate the effects of compound nylestriol tablet (CNT), containing nylestriol and levonorgestrel with a ratio of 1:0.3) and its components on the osteoporotic rat model induced by retinoic acid (RA) and ovariectomy (OVX).

METHODS:

We randomly divided 144 female SD rats (aged 7-month-old) into 12 groups (12 in each). In addition, 120 female SD rats aged 4-month were randomly divided into 10 groups (10 in each). Three dosage levels of CNT (0.039, 0.117 and 0.39 mg/kg body weight, daily), nylestriol (NYL, 0.30, 0.09 and 0.03 mg/kg body weight, daily) and levonorgestrel (LEV, 0.09, 0.027 and 0.009 mg/kg body weight, daily) were designed to prevent the bone loss of the osteoporotic rat model induced by RA and OVX respectively. Serum total calcium (Ca), phosphate (Pi), ALP, triglyceride (TG), total cholesterol (TC), HDL-C, total body BMD, isolated left femoral BMD and femoral Ca, and Pi after ashing were determined.

RESULTS:

Osteoporotic models could be induced by RA (70 mg/kg body weightdaily given intragastrically for 14 days) or by ovariectomy; CNT and its components had the preventive effects on bone loss in both models; The preventive effect of CNT was dose-dependent and stronger than its components; Many observed markers showed us the levels of high and moderate CNT given to be the strongest, corresponding to an optimal choice of the moderate dose CNT about one tablet per week in adult women.

CONCLUSIONS:

CNT therapy can prevent the bone loss of RA-induced and OVX-induced osteoporotic rats, and its therapeutic and preventive effect is better than its components used alone.

• Authors: Shi-Ping Liu, Er-Yuan Liao, Xian-Ping Wu, Xiang-Hang Luo, Huan-De Li, Ze-Neng Chen, Ru-Chun Dai, Rui-Yuan Sun, Xi-Jiu Liu
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 29 Issue 4 Pg. 405-9 (Aug 2004) ISSN: 1672-7347 [Print] China
• PMID: 16134591 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drug Combinations
• Tablets
• Phosphorus
• Tretinoin
• Levonorgestrel
• nylestriol
• Alkaline Phosphatase
• Quinestrol
• Calcium

Topics

• Alkaline Phosphatase (blood)
• Animals
• Calcium (blood)
• Drug Combinations
• Female
• Levonorgestrel (therapeutic use)
• Osteoporosis (drug therapy, etiology)
• Ovariectomy
• Phosphorus (blood)
• Quinestrol (analogs & derivatives, therapeutic use)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Tablets
• Tretinoin