Abstract | BACKGROUND AND OBJECTIVE: METHODS: In a prospective animal study 10 fasted, anaesthetized, mechanically ventilated and multi-catheterized pigs (20.6 +/- 1.3 kg) were investigated over a period of 8 h. Sepsis was induced by faecal peritonitis (1 g kg(-1) body weight faeces, n = 5) and compared to controls (n = 5). The animals received 6% hydroxyethyl starch 200/0.5 to maintain a central venous pressure of 12 mmHg. To quantify capillary leak syndrome, albumin escape rate was measured using 99mTc-labelled human serum albumin. Plasma levels of terminal complement complex were measured in a double antibody immunoassay (neoepitope-specific MoAb aE 11 as catching antibody). Immunohistological studies of renal specimens were performed to detect terminal complement complex deposition. RESULTS: Albumen escape rate increased in septic animals (+ 52%) compared to controls (+ 3%, P < 0.05). Plasma levels of terminal complement complex decreased during the study period in both groups. In septic animals this finding was accompanied by a significant deposition of terminal complement complex in renal specimens (P < 0.05). CONCLUSION:
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Authors | T Schuerholz, M Leuwer, M Cobas-Meyer, B Vangerow, F Kube, M Kirschfink, G Marx |
Journal | European journal of anaesthesiology
(Eur J Anaesthesiol)
Vol. 22
Issue 7
Pg. 541-7
(Jul 2005)
ISSN: 0265-0215 [Print] England |
PMID | 16045145
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers
- Blood Proteins
- Complement C5
- Complement Membrane Attack Complex
- Oxygen
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Topics |
- Animals
- Biomarkers
- Blood Proteins
(metabolism)
- Capillary Leak Syndrome
(physiopathology)
- Complement Activation
(physiology)
- Complement C5
(metabolism)
- Complement Membrane Attack Complex
(metabolism)
- Erythrocyte Volume
(physiology)
- Female
- Hematocrit
- Hemodynamics
- Immunohistochemistry
- Kidney
(pathology)
- Laparotomy
- Male
- Oxygen
(blood)
- Plasma Volume
(physiology)
- Shock, Septic
(metabolism, pathology)
- Swine
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