[Effect of calmodulin antagonist EBB on invasion of human fibrosarcoma cell HT1080].

Abstract

OBJECTIVE:

To investigate the potential effect of EBB, a calmodulin antagonist, on invasion of human fibrosarcoma cells HT1080.

METHODS:

The antitumor effect of EBB was assessed by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Activities of matrix metalloproteinase (MMP)-2 and MMP-9 were measured by Zymogrophy analysis. The mRNA levels, of MMP-2, MMP-9, and tissue inhibitor of metalloproteinases (TIMP)-1 were evaluated by reverse transcriptionpolymerase chain reaction (RT-PCR). Transwell chamber assay was applied to measure the effect of EBB on the invasion of HT1080 cells.

RESULTS:

Calmodulin antagonist EBB inhibited the proliferation of HT1080 cells with an IC50 of (8.2 +/- 1.2) microg/ml. EBB down-regulated the activities of MMP-2 and MMP-9, and down-regulated the mRNA levels of MMP-2 and MMP-9, while up-regulated the mRNA levels of TIMP-1. The invasive ability of HT1080 cells was decreased to (31.13 +/- 2.265)%, (59.91 +/- 2.566)%, and (71.58 +/- 0.5960)% after exposure of the cells with 2, 5, and 10 microg/ml EBB, respectively.

CONCLUSION:

Treatment with calmodulin antagonist EBB is effective in suppressing tumor invasion. The possible mechanism is the down-regulation of MMPs.

• Authors: Bing Pan, Yuan Zhou, Jing Qi, Dong-sheng Xiong, Jie-wen Liu, Shu-ling Qi, Yan-hong Cheng, Chun-zheng Yang, Hui-fang Zhu
• Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 27 Issue 3 Pg. 311-4 (Jun 2005) ISSN: 1000-503X [Print] China
• PMID: 16038266 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Benzylisoquinolines
• Calmodulin
• RNA, Messenger
• O-(4-ethoxybutyl)berbamine
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Antineoplastic Agents (pharmacology)
• Benzylisoquinolines (pharmacology)
• Calmodulin (antagonists & inhibitors)
• Cell Line, Tumor
• Down-Regulation
• Fibrosarcoma (pathology)
• Humans
• Matrix Metalloproteinase 2 (biosynthesis, genetics)
• Matrix Metalloproteinase 9 (biosynthesis, genetics)
• Neoplasm Invasiveness
• RNA, Messenger (biosynthesis, genetics)