Abstract | BACKGROUND: METHODS AND MATERIALS: These six hormones were evaluated for their ability to decrease the number and/or proliferation of human small-cell lung cancer cells in culture for 24, 48, 72, and 96 h. RESULTS: Within 24 h, vessel dilator, LANP, kaliuretic peptide, ANP and their intracellular mediator cyclic GMP, each at 1 microM, decreased the number of small-cell lung cancer cells by 63% (P < 0.001), 21% (P < 0.05), 30% (P < 0.05), 39% (P < 0.05), and 31% (P < 0.05), respectively. There was no proliferation in the 3 days following this decrease in cell number. These same hormones decreased DNA synthesis 68% to 82% (P < 0.001). Brain natriuretic peptide and CNP did not decrease the number of small-cell lung cancer cells or inhibit their DNA synthesis at 1 microM or 10 microM concentrations. Dose-response curves revealed that at 100 microM, the vessel dilator decreased 92% of the cancer cells in 24 h while BNP had no effect, but CNP caused a 39% decrease. Western blots revealed that the natriuretic peptide receptors A- and C- were present in these cancer cells. CONCLUSIONS:
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Authors | B A Vesely, S Song, J Sanchez-Ramos, S R Fitz, A A Alli, S M Solivan, W R Gower Jr, D L Vesely |
Journal | European journal of clinical investigation
(Eur J Clin Invest)
Vol. 35
Issue 6
Pg. 388-98
(Jun 2005)
ISSN: 0014-2972 [Print] England |
PMID | 15948900
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Protein Precursors
- Natriuretic Peptide, Brain
- Natriuretic Peptide, C-Type
- Atrial Natriuretic Factor
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Topics |
- Adenocarcinoma
(drug therapy, metabolism, pathology)
- Atrial Natriuretic Factor
(analysis)
- Blotting, Western
- Carcinoma, Small Cell
(drug therapy, metabolism, pathology)
- Cell Proliferation
(drug effects)
- Humans
- Lung Neoplasms
(drug therapy, metabolism, pathology)
- Natriuretic Peptide, Brain
(pharmacology)
- Natriuretic Peptide, C-Type
(pharmacology)
- Protein Precursors
(pharmacology)
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