Abstract | INTRODUCTION: METHODS: Male BALB/c mice were given orally 200 microl of water containing arsenic in a dose of 50, 100, and 150 mug/mouse/day for 6 days a week (experimental group) or arsenic-free water (<0.01 microg/l, control group) for 3, 6, 9 and 12 months. Hepatic glutathione (GSH), protein sulfhydryl (PSH), glutathione peroxidase (GPx), Catalase, lipid peroxidation (LPx), protein carbonyl (PC), interleukin (IL-6), tumor necrosis factor ( TNF-alpha), arsenic and collagen content in the liver were estimated from sacrificed animals. RESULTS: Significant increase of lipid peroxidation and protein oxidation in the liver associated with depletion of hepatic thiols (GSH, PSH), and antioxidant enzymes (GPx, Catalase) occurred in mice due to prolonged arsenic exposure in a dose-dependent manner. Significant elevation of hepatic collagen occurred at 9 and 12 months in all the groups associated with significant elevation of TNF-alpha and IL-6. However, arsenic level in the liver increased progressively from 3 months onwards. There was a positive correlation between the hepatic arsenic level and collagen content (r = 0.8007), LPx (r = 0.779) and IL-6 (r = 0.7801). Further, there was a significant negative correlation between GSH and TNF-alpha (r = -0.5336)) and LPx (r = -0.644). CONCLUSION: Increasing dose and duration of arsenic exposure in mice cause progressive increase of oxystress and elevation of cytokines associated with increasing level of collagen in the liver.
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Authors | Subhankar Das, Amal Santra, Sarbari Lahiri, D N Guha Mazumder |
Journal | Toxicology and applied pharmacology
(Toxicol Appl Pharmacol)
Vol. 204
Issue 1
Pg. 18-26
(Apr 01 2005)
ISSN: 0041-008X [Print] United States |
PMID | 15781290
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Interleukin-6
- Tumor Necrosis Factor-alpha
- Collagen
- Glutathione
- Arsenic
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Topics |
- Animals
- Arsenic
(analysis, toxicity)
- Collagen
(biosynthesis)
- Dose-Response Relationship, Drug
- Glutathione
(metabolism)
- Interleukin-6
(biosynthesis)
- Lipid Peroxidation
- Liver
(drug effects, metabolism, pathology)
- Male
- Mice
- Mice, Inbred BALB C
- Oxidative Stress
- Toxicity Tests, Chronic
- Tumor Necrosis Factor-alpha
(biosynthesis)
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