To evaluate the feasibility of
radionuclide gene therapy, we investigated the effect of
sodium iodide symporter (NIS) gene transfection on the uptake of some beta- and gamma-emitters in human
anaplastic thyroid cancer. NIS gene was transfected into human anaplastic
cancer ARO cells using
liposome (ARO-N) and its expression was confirmed by
reverse transcriptase-polymerase chain reaction (RT-PCR).
Iodide uptake by ARO-N was 109 times higher than by ARO, and 99mTc and 188Re uptake by ARO-N were 21 and 47 times higher than by ARO, respectively. The half-lives of
radionuclides (125I, 99mTc, and 188Re) retention in the cells were about 12, 3 and 4 min, respectively. Biodistribution studies showed that ARO-N
tumors accumulated higher amounts of
radionuclides than ARO
tumors. The mean accumulations of 125I, 99mTc, and 188Re in ARO-N
tumors were 18.3 +/- 8.7, 14.6 +/- 7.1 and 23.2 +/- 3.5% injected dose per gram (ID/g) at 2 hours postinjection, respectively. Scintigraphic images of
tumor bearing mice using 131I, 99mTc, and 188Re allowed clear visualization of ARO-N
tumors. In summary, NIS gene transfection to a single
anaplastic thyroid cancer cell line efficiently triggered high
tumor uptake of radioiodines, 99mTc and 188Re. These results demonstrate the possibility of imaging and
therapy using NIS gene transfection in
anaplastic thyroid carcinoma, although the short retention time is considered the major impediment to be resolved for the successful implementation.