Clinical implications of expression of ETS-1 related to angiogenesis in metastatic lesions of ovarian cancers.

Abstract

OBJECTIVE:

ETS-1 has been identified as a proto-oncogene and a transcription factor for tumor angiogenesis, which is essential for the growth, invasion and metastasis of solid tumors. The aim is to investigate the clinical implications of ETS-1 expression in peritoneal metastatic lesions of ovarian cancers.

METHODS:

In primary tumors and peritoneal metastatic lesions from 30 patients with stage III ovarian cancers, ETS-1 histoscores and ets-1 mRNA levels were determined by immunohistochemistry and competitive RT-PCR-Southern blot analysis using recombinant RNA, respectively.

RESULTS:

Immunohistochemical staining revealed that ETS-1 was expressed in the cancer cells and vascular endothelial cells. ETS-1 histoscores in the endothelial cells and ets-1 mRNA levels were significantly (p < 0.05) increased in 20 of 30 peritoneal metastatic lesions of ovarian cancers. There was a significant correlation between microvessel counts (MVCs) and ETS-1 histoscores in the endothelial cells (p < 0.001) and between MVCs and ets-1 mRNA levels in the primary tumor and the peritoneal metastatic lesion of ovarian cancers (p < 0.001). Furthermore, the 24-month survival rate of patients with significantly increased ets-1 mRNA level (2/20, 10%) was significantly (p < 0.01) lower than that of patients with no change in the level (6/10, 60%) from the primary tumor to the peritoneal metastatic lesion.

CONCLUSIONS:

ETS-1 might be associated with peritoneal metastasis dominantly as an angiogenic mediator and additionally as an oncogene product to activate tumor invasion in ovarian cancers.

• Authors: Jiro Fujimoto, Ikumi Aoki, Hiroshi Toyoki, Sufia Khatun, Eriko Sato, Hideki Sakaguchi, Teruhiko Tamaya
• Journal: Oncology (Oncology) Vol. 66 Issue 5 Pg. 420-8 ( 2004) ISSN: 0030-2414 [Print] Switzerland
• PMID: 15331930 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ETS1 protein, human
• MAS1 protein, human
• Proto-Oncogene Mas
• Proto-Oncogene Protein c-ets-1
• Proto-Oncogene Proteins
• Proto-Oncogene Proteins c-ets
• Transcription Factors
• Factor VIII

Topics

• Adult
• Aged
• Factor VIII (analysis)
• Female
• Humans
• Immunohistochemistry
• Middle Aged
• Neoplasm Metastasis
• Neovascularization, Pathologic
• Ovarian Neoplasms (blood supply, metabolism)
• Peritoneal Neoplasms (blood supply, metabolism, secondary)
• Proto-Oncogene Mas
• Proto-Oncogene Protein c-ets-1
• Proto-Oncogene Proteins (metabolism)
• Proto-Oncogene Proteins c-ets
• Survival Rate
• Transcription Factors (metabolism)