Abstract | AIM: To study the differential expression of proteins in normal and cancerous gastric tissues, and further identify new molecular markers for diagnosis and prognosis of gastric carcinoma, as well as develop new therapeutic targets of the disease. METHODS: Matched pairs of tissues from 6 gastric cancer patients were analyzed for their two-dimensional electrophoresis (2DE) profiles. Soluble fraction proteins from human normal and cancerous gastric tissue were separated in the first dimension by isoelectric focusing on immobilized pH gradient (IPG, pH3-10) strips, and by 125 g/L sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in the second dimension with silver nitrate staining. Protein differential expression was analyzed by use of image analysis software to find out candidates for gastric cancer-associated proteins. RESULTS:
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Authors | Kai-Juan Wang, Run-Tian Wang, Jian-Zhong Zhang |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 10
Issue 15
Pg. 2179-83
(Aug 01 2004)
ISSN: 1007-9327 [Print] United States |
PMID | 15259061
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Neoplasm Proteins
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Topics |
- Biomarkers, Tumor
(analysis)
- Carcinoma
(chemistry)
- Electrophoresis, Gel, Two-Dimensional
- Humans
- Neoplasm Proteins
(analysis)
- Reproducibility of Results
- Stomach Neoplasms
(chemistry)
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