AIM-2: a novel tumor antigen is expressed and presented by human glioma cells.

Abstract

Antigen isolated from Immunoselected Melanoma-2 (AIM-2) was recently identified using melanoma-reactive CD8 T cells. AIM-2 antigen is expressed in a wide variety of tumor types, including neuroectodermal tumors, as well as breast, ovarian and colon carcinomas. In this study, we analyzed AIM-2 expression in glioblastoma multiforme (GBM) in primary cultured cells and established GBM cell lines. We found that the primary GBM cell lines expressed 88.4% and 93.0% of non-spliced and spliced AIM-2, respectively. Five out of seven of the established GBM cell lines expressed both non-spliced and spliced AIM-2. Furthermore, the C9 CTL clone, which is specific for AIM-2 peptide (RSDSGQQARY), efficiently recognized GBM tumor cells in an antigen-specific and HLA-A1 restricted manner. IFN-gamma treatment of the GBM tumor cells dramatically increased HLA-A1 expression levels and, consequently, increased CTL recognition of the treated tumor cells. More importantly, seven out of 12 HLA-A1 and AIM-2 positive patients from our dendritic cell clinical trial generated AIM-2 specific CTL activity in their PBMC after vaccinations. These data indicate that AIM-2 could be used as a tumor antigen target for monitoring vaccine trials or to develop antigen specific active immunotherapy for glioma patients.

• Authors: Gentao Liu, John S Yu, Gang Zeng, Dong Yin, Dong Xie, Keith L Black, Han Ying
• Journal: Journal of immunotherapy (Hagerstown, Md. : 1997) (J Immunother) 2004 May-Jun Vol. 27 Issue 3 Pg. 220-6 ISSN: 1524-9557 [Print] United States
• PMID: 15076139 (Publication Type: Journal Article)

Chemical References

• AIM2 protein, human
• Antigens, Neoplasm
• DNA, Complementary
• DNA-Binding Proteins
• HLA-A1 Antigen
• Nuclear Proteins
• Peptides
• RNA, Messenger
• RNA
• Interferon-gamma

Topics

• Antigens, Neoplasm
• Brain Neoplasms (metabolism)
• Cell Line, Tumor
• DNA, Complementary (metabolism)
• DNA-Binding Proteins
• Dendritic Cells (cytology, immunology)
• Enzyme-Linked Immunosorbent Assay
• Glioblastoma (metabolism)
• Glioma (metabolism)
• HLA-A1 Antigen (chemistry)
• Humans
• Immunotherapy (methods)
• Interferon-gamma (metabolism)
• Leukocytes, Mononuclear (metabolism)
• Nuclear Proteins (biosynthesis, chemistry)
• Peptides (chemistry)
• RNA (chemistry)
• RNA, Messenger (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• T-Lymphocytes, Cytotoxic (metabolism)
• Time Factors
• Up-Regulation