Amadori-glycated phosphatidylethanolamine induces angiogenic differentiations in cultured human umbilical vein endothelial cells.

Abstract

Glycation has been implicated in the endothelial dysfunction that contributes to both diabetes- and aging-associated vascular complications. The aim of the present study was to determine whether Amadori-glycated phosphatidylethanolamine (Amadori-PE), a lipid-linked glycation compound that is formed at an increased rate in hyperglycemic states, affected proliferation, migration and tube formation of cultured human umbilical vein endothelial cells (HUVEC). Amadori-PE at a low concentration of less than 5 microM significantly enhanced these three factors involved in angiogenesis. Furthermore, stimulation of HUVEC with Amadori-PE resulted in secretion of matrix metalloproteinase 2 (MMP-2), a pivotal enzyme in the initial step of angiogenesis. Our results demonstrated for the first time that Amadori-PE may be an important compound that promotes vascular disease as a result of its angiogenic activity on endothelial cells. We also demonstrated that MMP-2 is a primary mediator of Amadori-PE-driven angiogenesis.

• Authors: Jeong-Ho Oak, Kiyotaka Nakagawa, Shinichi Oikawa, Teruo Miyazawa
• Journal: FEBS letters (FEBS Lett) Vol. 555 Issue 2 Pg. 419-23 (Dec 04 2003) ISSN: 0014-5793 [Print] England
• PMID: 14644453 (Publication Type: Journal Article)

Chemical References

• Phosphatidylethanolamines
• Vascular Endothelial Growth Factors
• Collagenases

Topics

• Cell Division (drug effects)
• Cell Movement (drug effects)
• Cells, Cultured
• Collagenases (metabolism)
• Endothelium, Vascular (cytology, drug effects, metabolism)
• Fibroblasts (drug effects, metabolism)
• Glycosylation
• Humans
• Neovascularization, Pathologic (drug therapy)
• Phosphatidylethanolamines (chemistry, pharmacology)
• Umbilical Veins (cytology)
• Vascular Endothelial Growth Factors (metabolism)