Abstract | BACKGROUND: Hepatitis C virus infection is a great issue in China; however, there is very little information on genotyping investigations based on sequence variability in the 5'untranslated ( 5'UTR) reported. The present study was to define the sequence variability based on the sequence divergences of the 5'UTR of the virus. METHODS: Sequences of 91 isolates from patients with chronic hepatitis C from Yunnan, southwest China, were sequenced and genotypes were defined according to the sequence divergences of the 5'UTR of the virus. RESULTS: Eighty-six isolates were classified into 3 clades (previously termed groups or major types) by the methods proposed by Chan et al in 1992 and phylogenetic analysis based on nucleotide sequence divergences within the 5'UTR. Fifty-six percent of the isolates were classified into clade 3, 35% into clade 1, and 34.9% into clade 2. New genotypes 1f, 2h, 3h and 3i were defined. In addition, 3 novel sequences were discovered, respectively with an 18-nt sequence deletion (corresponding to nucleotide position -173 to -156), a 28-nt sequence insertion, and a 40-nt sequence insertion, between -56 and -55. Of these isolates, 56% possessed a "G" at position -66 in place of the "T" that is present in all previously reported sequences. CONCLUSIONS: These HCV variants, evolved or remained in this area, may be of great significance in diagnosis and treatment of hepatitis C patients.
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Authors | Yuan-Ding Chen, Ming-Ying Liu, Wen-Lin Yu, Jia-Qi Li, Mei Peng, Qing Dai, Jun Wu, Xiao Liu, Zhen-Quan Zhou |
Journal | Hepatobiliary & pancreatic diseases international : HBPD INT
(Hepatobiliary Pancreat Dis Int)
Vol. 1
Issue 4
Pg. 541-52
(Nov 2002)
ISSN: 1499-3872 [Print] Singapore |
PMID | 14607684
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 5' Untranslated Regions
- DNA Transposable Elements
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Topics |
- 5' Untranslated Regions
(genetics)
- Adolescent
- Adult
- Aged
- Base Sequence
- Child
- China
- Cloning, Molecular
- DNA Transposable Elements
- Female
- Gene Deletion
- Genetic Variation
- Genotype
- Hepacivirus
(genetics)
- Hepatitis C, Chronic
(epidemiology, virology)
- Humans
- Incidence
- Male
- Middle Aged
- Molecular Sequence Data
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