Human leukocyte formin: a novel protein expressed in lymphoid malignancies and associated with Akt.
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| Abstract |
The very large family of Formin proteins is involved in processes such as morphogenesis, embryonic differentiation, cell polarity, and cytokinesis. A novel human gene from the Formin family, denominated human leukocyte formin gene, was cloned. The cDNA of the gene was determined to be 3959bp long with an open reading frame of 3302bp and computational analysis located this gene on chromosome 17, suggesting that it is composed of 27 exons. Northern blot analysis revealed a restricted expression of mRNA in the thymus, spleen, and peripheral blood leukocytes in normal human tissues. Western blot analysis demonstrated that the protein encoded by this gene is overexpressed in lymphoid malignancies; cancer cell lines and peripheral blood leukocyte from chronic lymphocytic leukemia (CLL) patients. Furthermore, the human leukocyte formin protein was observed to associate with Akt, a critical survival regulator in many different cell types.
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| Authors | Patricia M Bergamo Favaro, Samuel de Souza Medina, Fabíola Traina, Daniela Sanchez Bassères, Fernando Ferreira Costa, Sara Teresinha Olalla Saad |
| Journal | Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 311 Issue 2 Pg. 365-71 (Nov 14 2003) ISSN: 0006-291X [Print] United States |
| PMID | 14592423 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't) |
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