Abstract |
Previous reports suggest that the hypothalamic growth hormone-releasing hormone (GHRH) promotes sleep, especially non-rapid-eye-movement sleep (NREMS). To evaluate the role of endogenous GHRH in sleep regulation, the effects of antibodies to rat GHRH (GHRH-ab) were studied on normal sleep, brain temperature (Tbr), and GH secretion in experiment I and on enhanced sleep after sleep deprivation in experiment II. In experiment I, affinity-purified GHRH- ab (50 and 200 micrograms) raised in goats and a control goat immunoglobulin G ( IgG) preparation were injected intracerebroventricularly (icv) in rats 1 h before the onset of the light cycle, and sleep-wake activity and Tbr were recorded for the next 12 or 23 h. Both doses of GHRH-ab suppressed NREMS and REMS throughout the light cycle. Sleep durations at night were normal. Electroencephalographic (EEG) slow-wave activity, characterized by EEG slow-wave amplitudes, was reduced after GHRH-ab during both the light and the dark cycles. Plasma GH concentrations measured 6-12 h after injection of GHRH-ab (200 micrograms) were diminished. Both the control IgG and GHRH-ab elicited fever. In experiment II, the sleep-wake activity and Tbr of rats were recorded for 24 h in three experimental conditions: base-line with icv injection of IgG, 3-h sleep deprivation with icv IgG injection, and 3-h sleep deprivation with icv GHRH-ab (200 micrograms). After sleep deprivation (+ IgG), a prompt increase in EEG slow-wave activity (power density analysis) and late increases in NREMS and REMS durations were found.(ABSTRACT TRUNCATED AT 250 WORDS)
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Authors | F Obál Jr, L Payne, M Opp, P Alföldi, L Kapás, J M Krueger |
Journal | The American journal of physiology
(Am J Physiol)
Vol. 263
Issue 5 Pt 2
Pg. R1078-85
(Nov 1992)
ISSN: 0002-9513 [Print] United States |
PMID | 1443226
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies
- Growth Hormone
- Growth Hormone-Releasing Hormone
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Topics |
- Animals
- Antibodies
(administration & dosage, immunology)
- Body Temperature
- Brain
(physiology)
- Growth Hormone
(metabolism)
- Growth Hormone-Releasing Hormone
(immunology)
- Injections, Intraventricular
- Male
- Rats
- Rats, Sprague-Dawley
- Sleep
(physiology)
- Sleep Deprivation
(physiology)
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