Scrapie-associated tubulofilamentous particles in human Creutzfeldt-Jakob disease.

Abstract	Scrapie-associated fibrils (SAF) were demonstrated by a simple negative staining method for electron microscopy from fresh and frozen brains with naturally occurring human Creutzfeldt-Jakob disease (CJD). The findings confirm that SAF occur as an internal part of a larger three-layer particle. The two outer coats of SAF can be disrupted by detergent alone or can be digested in two stages by a combination of proteolytic enzymes and subsequent treatment with DNase and mung bean nuclease. Examination of thin sections from the cerebral cortex of brains from patients with CJD revealed the presence of 26-30-nm diameter tubulofilamentous particles, identical to those previously described in natural scrapie of sheep and bovine spongiform encephalopathy and also in experimentally induced scrapie in mice and hamsters and CJD-infected mice and chimpanzees. Thus, it would appear that the particles are not contaminants passaged in experimental animals.
Authors	H K Narang
Journal	Research in virology (Res Virol) 1992 Nov-Dec Vol. 143 Issue 6 Pg. 387-95 ISSN: 0923-2516 [Print] France
PMID	1363619 (Publication Type: Comparative Study, Journal Article)
Chemical References	DNA, Mitochondrial DNA, Single-Stranded DNA, Viral Membrane Glycoproteins Nerve Tissue Proteins PrPSc Proteins Prions Sialoglycoproteins PrP 27-30 Protein
Topics	Adult Aged Aged, 80 and over Alzheimer Disease (metabolism, pathology) Brain (microbiology, ultrastructure) Brain Chemistry Creutzfeldt-Jakob Syndrome (microbiology, pathology) DNA, Mitochondrial (isolation & purification) DNA, Single-Stranded (isolation & purification) DNA, Viral (isolation & purification) Humans Membrane Glycoproteins (isolation & purification, metabolism) Microscopy, Electron Middle Aged Models, Biological Negative Staining Nerve Tissue Proteins (isolation & purification, metabolism, ultrastructure) PrP 27-30 Protein PrPSc Proteins Prions (isolation & purification, metabolism, ultrastructure) Sialoglycoproteins (isolation & purification, metabolism, ultrastructure)

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