Molecular basis of constitutive production of basement membrane components. Gene expression profiles of Engelbreth-Holm-Swarm tumor and F9 embryonal carcinoma cells.

Abstract

Engelbreth-Holm-Swarm (EHS) tumors produce large amounts of basement membrane (BM) components that are widely used as cell culture substrates mimicking BM functions. To delineate the tissue/organ origin of the tumor and the mechanisms operating in the BM overproduction, a genome-wide expression profile of EHS tumor was analyzed using RIKEN cDNA microarrays containing approximately 40,000 mouse cDNA clones. Expression profiles of F9 embryonal carcinoma cells that produce laminin-1 and other BM components upon differentiation into parietal endoderm-like cells (designated F9-PE) were also analyzed. Hierarchical clustering analysis showed that the gene expression profiles of EHS and F9-PE were the most similar among 49 mouse tissues/organs in the RIKEN Expression Array Database, suggesting that EHS tumor is parietal endoderm-derived. Quantitative PCR analysis confirmed that not only BM components but also the machineries required for efficient production of BM components, such as enzymes involved in post-translational modification and molecular chaperones, were highly expressed in both EHS and F9-PE. Pairs of similar transcription factor isoforms, such as Gata4/Gata6, Sox7/Sox17, and Cited1/Cited2, were also highly expressed in both EHS tumor and F9-PE. Time course analysis of F9 differentiation showed that up-regulation of the transcription factors was associated with those of BM components, suggesting their involvement in parietal endoderm specification and overproduction of the BM components.

• Authors: Sugiko Futaki, Yoshitaka Hayashi, Megumi Yamashita, Ken Yagi, Hidemasa Bono, Yoshihide Hayashizaki, Yasushi Okazaki, Kiyotoshi Sekiguchi
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 278 Issue 50 Pg. 50691-701 (Dec 12 2003) ISSN: 0021-9258 [Print] United States
• PMID: 12968032 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Complementary
• Protein Isoforms

Topics

• Animals
• Basement Membrane (chemistry, physiology)
• Blotting, Northern
• Blotting, Western
• Cell Differentiation
• Cell Line, Tumor
• DNA, Complementary (metabolism)
• Endoderm (cytology)
• Mice
• Mice, Inbred C57BL
• Neoplasm Transplantation
• Oligonucleotide Array Sequence Analysis
• Phylogeny
• Polymerase Chain Reaction
• Protein Isoforms
• Time Factors