Abstract |
Central dopaminergic systems are implicated in schizophrenia and Parkinson's disease, and are known to be modulated by the endogenous tripeptide Pro-Leu-Gly-NH(2) (PLG or MIF-1, melanocyte-stimulating hormone release inhibiting factor-1). Differential display polymerase chain reaction (ddPCR) was utilized to identify genes that are regulated by protracted PLG treatment (20 mg/kg, i.p. for 28 days) in male Sprague-Dawley rats. A total of 2400 genes were screened and 3 down-regulated bands were identified in the PLG-treated samples. Sequencing analysis revealed a total of six unique cDNA species. One fragment possessed a high degree of homology with Mus musculus hnRNP-L ( protein L) mRNA (GenBank #AB009392) (termed PRG1: PLG regulated gene 1). Elongation of the PRG1 cDNA, by RACE-PCR, provided an 835 bp sequence with 95% homology to AB009392 over a 743 bp span. Open reading frame analysis provided a putative amino acid sequence consistent with the identity of PRG1 as rat hnRNP-L. Northern hybridization experiments with PRG1 revealed a 2.3 kb mRNA species that was decreased by 65% in the PLG-treated tissue. Western blot analysis revealed significantly decreased hnRNP-L levels in the striatum and pre-frontal cortex (but not the nucleus accumbens) by 71 and 61%, respectively of PLG-treated animals. The identification of altered expression of hnRNP-L following PLG treatment provides insight into the long-term effects of PLG and may provide insight into its molecular mechanism of action.
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Authors | Willard J Costain, Ram K Mishra |
Journal | Peptides
(Peptides)
Vol. 24
Issue 1
Pg. 137-46
(Jan 2003)
ISSN: 0196-9781 [Print] United States |
PMID | 12576095
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Copyright | Copyright 2002 Elsevier Science Inc. |
Chemical References |
- DNA, Complementary
- Heterogeneous-Nuclear Ribonucleoprotein L
- MSH Release-Inhibiting Hormone
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Topics |
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Blotting, Western
- Corpus Striatum
(drug effects, metabolism)
- DNA, Complementary
- Heterogeneous-Nuclear Ribonucleoprotein L
(genetics)
- MSH Release-Inhibiting Hormone
(pharmacology)
- Male
- Molecular Sequence Data
- Polymerase Chain Reaction
- Prefrontal Cortex
(drug effects, metabolism)
- Rats
- Rats, Sprague-Dawley
- Sequence Homology, Amino Acid
- Sequence Homology, Nucleic Acid
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