Anti-implantation effect of a carbamate fungicide mancozeb in albino mice.

Abstract

Mancozeb, an organocarbamate fungicide, was administered to examine the effect on implantation at doses of 18, 24, 30 and 36 mg/kg body weight/d to normal virgin swiss albino mice for 8 days to pregnant mice. The vaginal smear and body weight of the mice were recorded daily and mice were sacrificed on 9th day of pregnancy. There was a complete inhibition of implantation in 36 mg mancozeb treated mice with 100% pre-implantation loss. There was a partial inhibition of implantation in 24 and 30 mg mancozeb treated mice with 53.44 and 90.16% pre-implantation loss respectively. However, implantation was not affected in 18 mg mancozeb treated mice with 4.92% pre-implantation loss when compared to oil treated controls. To study the temporal effect, the effective dose of 36 mg/kg body weight/d mancozeb was administered orally for 3 and 5 days and on day 3 only. There was a complete inhibition of implantation in 5 days treated mice with 100% pre-implantation loss and partial inhibition of implantation of 3 days treated mice with 75% pre-implantation loss. However, implantation was not affected in mice treated on day 3 only with 1.63% pre-implantation loss when compared to control mice. There was a significant decrease in the diestrus phase with the result there was a concomitant increase in the estrus phase and there was a significant decrease in the uterus weight with 24, 30 and 36 mg and for 3 and 5 days with 36 mg mancozeb treatment. Inhibition of implantation by mancozeb may be due to hormonal imbalance or its toxic effects.

• Authors: Bharati B Bindali, Basappa B Kaliwal
• Journal: Industrial health (Ind Health) Vol. 40 Issue 2 Pg. 191-7 (Apr 2002) ISSN: 0019-8366 [Print] Japan
• PMID: 12064561 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fungicides, Industrial
• Maneb
• mancozeb
• Zineb

Topics

• Animals
• Dose-Response Relationship, Drug
• Embryo Implantation (drug effects)
• Estrus
• Female
• Fungicides, Industrial (adverse effects)
• Maneb (adverse effects)
• Mice
• Pregnancy
• Zineb (adverse effects)