[Telomerase activity in lung cancer and adjacent peritumoral tissues determined by TRAP-SYBR green assay].

Abstract

OBJECTIVE:

To develop methods for the qualitative analysis of telomerase activity by TRAP-SYBR Green assay and study telomerase activity in lung cancer and adjacent peritumoral tissues, and to explore the possible association with expression of P53.

METHODS:

Thirty six lung cancer tissues and 36 adjacent peritumoral tissues, 8 lung benign lesion tissues and A549 lung adenoma cell line, were examined for telomerase activity by TRAP-SYBR Green assay. The 36 lung cancer tissue samples were also examined for P53 protein expression by immunohistochemical staining.

RESULTS:

Telomerase activity in 5 cells of A549 lung cancer cell line was detected by TRAP-SYBR Green assay. Thirty of 36 (83.3%) lung cancer specimens, seven of 36 (19.4%) adjacent peritumoral tissues, three of 8 (37.5%) lung benign lesion tissues were positive for telomerase activity. Telomerase activity of lung cancer did not correlate with pathologic type, tumor size and clinical stage. P53 was over-expressed in 24 of the 30 (80%) lung cancer samples with telomerase activity. A significant correlation was found between telomerase activity and accumulation of P53 proteins (r = 00.60, P < 0.01).

CONCLUSION:

TRAP-SYBR Green assay may be a sensitive, fast and convenient method for qualitative analysis of telomerase activity, which is useful in diagnosis lung neoplasms. The relationship between telomerase activity and P53 accumulation needs further study.

• Authors: M He, S Xu, X Wang
• Journal: Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] (Zhonghua Yu Fang Yi Xue Za Zhi) Vol. 35 Issue 5 Pg. 301-4 (Sep 2001) ISSN: 0253-9624 [Print] China
• PMID: 11769626 (Publication Type: English Abstract, Journal Article)

Chemical References

• Fluorescent Dyes
• Organic Chemicals
• SYBR Green II
• Tumor Suppressor Protein p53
• Telomerase

Topics

• Fluorescent Dyes
• Humans
• Immunohistochemistry (methods)
• Lung (enzymology)
• Lung Neoplasms (diagnosis, enzymology)
• Organic Chemicals
• Staining and Labeling (methods)
• Telomerase (analysis)
• Tumor Cells, Cultured
• Tumor Suppressor Protein p53 (analysis)