Overexpression of an mRNA-binding protein in human colorectal cancer.

Abstract

A Coding Region instability Determinant-Binding Protein (CRD-BP) binds in vitro to c-myc mRNA and appears to stabilize the mRNA. The CRD-BP gene is amplified in one-third of human breast cancer cases, and the CRD-BP appears to be an oncofetal protein. To analyse CRD-BP expression in human cancer tissue, paired extracts of cancer and normal colon specimens from 21 patients were analysed by immunoblotting and/or reverse transcriptase-polymerase chain reaction. Seventeen cancer specimens out of 21 (81%) were positive for CRD-BP expression by one or both assays. With one exception, normal colon specimens were negative for CRD-BP expression; specimens of inflammatory bowel and a villous adenoma also had no detectable CRD-BP. The lack of CRD-BP expression in normal colon did not result from indiscriminate protein or RNA degradation. c-myc mRNA levels appeared to be elevated in tumor specimens. We conclude that the CRD-BP is scarce or absent from normal colon but is overexpressed in colorectal cancer. The CRD-BP might be a novel human tumor marker.

• Authors: J Ross, I Lemm, B Berberet
• Journal: Oncogene (Oncogene) Vol. 20 Issue 45 Pg. 6544-50 (Oct 04 2001) ISSN: 0950-9232 [Print] England
• PMID: 11641779 (Publication Type: Case Reports, Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Biomarkers, Tumor
• IGF2BP1 protein, human
• Proto-Oncogene Proteins c-myc
• RNA, Messenger
• RNA, Neoplasm
• RNA-Binding Proteins

Topics

• Adult
• Aged
• Biomarkers, Tumor (biosynthesis, genetics)
• Carcinoma (genetics, metabolism)
• Colon (metabolism)
• Colorectal Neoplasms (genetics, metabolism)
• Female
• Humans
• Male
• Middle Aged
• Proto-Oncogene Proteins c-myc (biosynthesis, genetics)
• RNA, Messenger (biosynthesis)
• RNA, Neoplasm (biosynthesis)
• RNA-Binding Proteins (biosynthesis, genetics)
• Transcription, Genetic
• Up-Regulation