Comparison of the uptake and clearance of Tc-99m MIBI, Tl-201 and Ga-67 in drug-resistant lymphoma cell lines.

Abstract

Tc-99m sestamibi (MIBI) has been used as a tumor-seeking agent. However, its role in detecting lymphomas has not been widely investigated. The aim of the present study was to determine the uptake and clearance characteristics of Tc-99m MIBI in vincristine-resistant lymphoma cell lines. In addition, thallium-201 (Tl-201) and gallium-67 (Ga-67) uptake and clearance characteristics were evaluated for comparison with Tc-99m MIBI. Drug-resistant lymphoma cell lines (monocyte-like, histiocytic lymphoma, human; B-lymphoma cell line, American Burkitt lymphoma, lymphoblastoid, human; Hodgkin's disease, lymphoid, human) were selected by multistep vincristine treatment up to 50 nM. After incubation of the radiotracers, Tc-99m MIBI, Tl-201 and Ga-67, in medium for 0, 10, 20, 30, 60 or 120 min, the uptake and clearance of each radiotracer were measured in the drug-resistant lymphoma cell lines. In addition, P-glycoprotein expression was determined by immunohistochemical study. In a comparison of the three radiotracers, the uptake of Tc-99m MIBI was the greatest in the studied wild-type lymphoma cell lines. Tc-99m MIBI uptake was much lower in drug-resistant tumor cell lines than in non-resistant cell lines. On the other hand, the uptake characteristics of Tl-201 did not differ between drug-resistant and non-resistant cells. Immunohistochemistry analyses of Ab-1 or JSB indicated that tumor cells expressed MDR-1 protein in all three cell lines. Tc-99m MIBI is a good radiotracer for detecting drug resistance in lymphoma cell lines.

• Authors: S C Tsai, Y C Shiau, J J Wang, Y J Ho, C H Kao
• Journal: Cancer letters (Cancer Lett) Vol. 171 Issue 2 Pg. 147-52 (Oct 10 2001) ISSN: 0304-3835 [Print] Ireland
• PMID: 11520598 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ATP Binding Cassette Transporter, Subfamily B, Member 1
• Antineoplastic Agents, Phytogenic
• Gallium Radioisotopes
• Radiopharmaceuticals
• Thallium Radioisotopes
• Vincristine
• Technetium Tc 99m Sestamibi

Topics

• ATP Binding Cassette Transporter, Subfamily B, Member 1 (biosynthesis, metabolism)
• Antineoplastic Agents, Phytogenic (pharmacology)
• Burkitt Lymphoma (metabolism)
• Drug Resistance, Neoplasm
• Gallium Radioisotopes (pharmacokinetics)
• Hodgkin Disease (metabolism)
• Humans
• Lymphoma (metabolism)
• Radiopharmaceuticals (pharmacokinetics)
• Technetium Tc 99m Sestamibi (pharmacokinetics)
• Thallium Radioisotopes (pharmacokinetics)
• Tumor Cells, Cultured
• U937 Cells
• Vincristine (pharmacology)