Application of CARD-ISH for Assessment of Numerical Chromosome Aberrations in Interphase Nuclei of Human Tumor Cells.

Abstract

In this study based on the study of a centromeric DNA probe specific for chromosome 8 the authors standardized a method for the enzymatic detection of specific chromosome copy numbers on interphase nuclei from tumor tissue samples. Since the in situ hybridization (ISH) of chromosome 8 specific probe was revealed with a catalyzed reporter deposition (CARD), which allows a high amplification of the hybridization signal, the method was designated as CARD-ISH. This method has been standardized on interphase nuclei isolated from clinical samples of pituitary adenomas, as well as on human normal lymphocytes. On the same samples, they also evaluated chromosome 8 copy number distribution by FISH. Comparison between CARD-ISH and FISH results showed no significant differences between the two methods, proposing CARD-ISH as a reliable alternative to FISH for chromosome numerical aberration assessment in laboratories that do not have specific facilities for epifluorescence microscopy or cytogenetics and that need a long-term storage of slides that had been used for diagnostic purposes. Int J Surg Pathol 8(3):201-206, 2000

• Authors: Laura Buonamici, Massimo Serra, Luisa Losi, Vincenzo Eusebi
• Journal: International journal of surgical pathology (Int J Surg Pathol) Vol. 8 Issue 3 Pg. 201-206 (Jul 2000) ISSN: 1940-2465 [Electronic] United States
• PMID: 11493990 (Publication Type: Journal Article)