Identification of the intracellular polyhydroxyalkanoate depolymerase gene of Paracoccus denitrificans and some properties of the gene product.

Abstract

Paracoccus denitrificans degraded poly(3-hydroxybutyrate) (PHB) in the cells under carbon source starvation. Intracellular poly(3-hydroxyalkanoate) (PHA) depolymerase gene (phaZ) was identified near the PHA synthase gene (phaC) of P. denitrificans. Cell extract of Escherichia coli carrying lacZ--phaZ fusion gene degraded protease-treated PHB granules. Reaction products were thought to be mainly D(--)-3-hydroxybutyrate (3HB) dimer and 3HB oligomer. Diisopropylfluorophosphonate and Triton X-100 exhibited an inhibitory effect on the degradation of PHB granules. When cell extract of the recombinant E. coli was used, Mg(2+) ion inhibited PHB degradation. However, the inhibitory effect by Mg(2+) ion was not observed using the cell extract of P. denitrificans.

• Authors: D Gao, A Maehara, T Yamane, S Ueda
• Journal: FEMS microbiology letters (FEMS Microbiol Lett) Vol. 196 Issue 2 Pg. 159-64 (Mar 15 2001) ISSN: 0378-1097 [Print] England
• PMID: 11267773 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Bacterial
• Hydroxybutyrates
• Indicators and Reagents
• Polyesters
• Recombinant Proteins
• Carboxylic Ester Hydrolases
• poly(3-hydroxyalkanoic acid) depolymerase
• Magnesium

Topics

• Amino Acid Sequence
• Base Sequence
• Biodegradation, Environmental
• Carboxylic Ester Hydrolases (genetics, isolation & purification)
• Cloning, Molecular
• DNA, Bacterial
• Escherichia coli
• Genes, Bacterial
• Hydroxybutyrates (metabolism)
• Indicators and Reagents
• Magnesium (metabolism)
• Molecular Sequence Data
• Paracoccus denitrificans (enzymology, genetics)
• Polyesters (metabolism)
• Recombinant Proteins (chemistry, metabolism)