The gene for hepatocyte nuclear factor (HNF)-4alpha is activated by glucocorticoids and glucagon, and repressed by insulin in rat liver.

Abstract

The gene for a transcription factor hepatocyte nuclear factor-4alpha (HNF-4alpha) is responsible for maturity-onset diabetes of the young, type 1. We examined hormonal regulation of the HNF-4alpha gene in the liver. Stimulation of primary-cultured rat hepatocytes with dexamethasone or glucagon led to induction of HNF-4alpha mRNA, being antagonized by insulin. In the liver of streptozotocin-induced diabetic rat, mRNA and protein levels for HNF-4alpha were elevated, and were normalized by insulin treatment. Therefore, HNF-4alpha in the liver is likely to be involved in the regulation of glucose metabolism in response to these hormones.

• Authors: S Oyadomari, F Matsuno, S Chowdhury, T Kimura, K Iwase, E Araki, M Shichiri, M Mori, M Takiguchi
• Journal: FEBS letters (FEBS Lett) Vol. 478 Issue 1-2 Pg. 141-6 (Jul 28 2000) ISSN: 0014-5793 [Print] England
• PMID: 10922486 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA-Binding Proteins
• Hepatocyte Nuclear Factor 4
• Insulin
• Insulin Antagonists
• Phosphoproteins
• RNA, Messenger
• Transcription Factors
• Dexamethasone
• Glucagon
• Glucose

Topics

• Animals
• Blotting, Western
• Cells, Cultured
• DNA-Binding Proteins
• Dexamethasone (antagonists & inhibitors, pharmacology)
• Diabetes Mellitus, Experimental (chemically induced, genetics, metabolism)
• Gene Expression Regulation (drug effects)
• Glucagon (antagonists & inhibitors, pharmacology)
• Glucose (metabolism)
• Hepatocyte Nuclear Factor 4
• Insulin (pharmacology)
• Insulin Antagonists (pharmacology)
• Liver (cytology, drug effects, metabolism, pathology)
• Male
• Phosphoproteins (genetics, metabolism)
• RNA, Messenger (genetics, metabolism)
• Rats
• Rats, Wistar
• Transcription Factors (genetics, metabolism)
• Transcriptional Activation (drug effects)