Abstract | BACKGROUND/PURPOSE: METHODS: A CDH model was induced in pregnant rats after administration of nitrofen. Dexamethasone (Dex; 0.25 mg/kg) was given intraperitoneally on day 18.5 and 19.5 of gestation (term, day 22). Cesarean section was performed on day 21 of gestation. mRNA was extracted from left lung and reverse transcription-polymerase chain reaction (RT-PCR) was performed to evaluate mRNA expression of each growth factors. Relative levels of mRNA were expressed as a ratio of the band density divided by that of beta-actin, a housekeeping gene known to be expressed at a constant level. RESULTS: Relative mRNA levels of bFGF and TGF-beta1 were decreased significantly in CDH lung compared with controls. Antenatal Dex treatment up-regulated gene expression of bFGF, PDGF, and TGF-beta1 in the hypoplastic CDH lung. CONCLUSIONS: The authors' findings suggest that decreased gene expression of bFGF, PDGF, and TGF-beta1 in the CDH lung may suppress lung growth and development. Increased gene expression of bFGF, PDGF, and TGF-beta1 in Dex-treated lung suggests that antenatal glucocorticoid administration may accelerate fetal lung growth by up-regulating these growth factors.
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Authors | T Oue, H Shima, Y Taira, P Puri |
Journal | Journal of pediatric surgery
(J Pediatr Surg)
Vol. 35
Issue 1
Pg. 109-12
(Jan 2000)
ISSN: 0022-3468 [Print] United States |
PMID | 10646786
(Publication Type: Journal Article)
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Chemical References |
- Glucocorticoids
- Growth Substances
- Phenyl Ethers
- Platelet-Derived Growth Factor
- RNA, Messenger
- Transforming Growth Factor beta
- Fibroblast Growth Factor 2
- Dexamethasone
- nitrofen
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Topics |
- Animals
- Dexamethasone
(administration & dosage)
- Fibroblast Growth Factor 2
(genetics, metabolism)
- Gene Expression
- Glucocorticoids
(administration & dosage)
- Growth Substances
(metabolism)
- Hernia, Diaphragmatic
(chemically induced, embryology, metabolism)
- Lung
(embryology, metabolism)
- Phenyl Ethers
- Platelet-Derived Growth Factor
(genetics, metabolism)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Reverse Transcriptase Polymerase Chain Reaction
- Transforming Growth Factor beta
(genetics, metabolism)
- Up-Regulation
(drug effects)
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