Suppression of the neoplastic phenotype by transfection of phospholipase C beta 3 to neuroendocrine tumor cells.

Abstract

The expression of phospholipase C beta 3 (PLCB3) is low or absent in several neuroendocrine neoplasias. To investigate the role of PLCB3 in the neuroendocrine tumorigenesis, we transfected a PLCB3 construct to three neuroendocrine tumor cell lines with a low PLCB3 expression. The growth rate and tumorigenicity were assessed in vitro by [3H]thymidine incorporation and cell counting, in vivo, by xenografting to nude mice. In vitro, PLCB3 expressing clones showed a significant growth inhibition. The tumor weight was reduced for one of the two xenografted PLCB3-transfected cell lines and in both, a reduced number of proliferating (Ki-67 positive) cells was observed. This study implies an essential role for PLCB3 in the neuroendocrine tumorigenesis.

• Authors: P Stålberg, S Wang, C Larsson, G Weber, K Oberg, A Gobl, B Skogseid
• Journal: FEBS letters (FEBS Lett) Vol. 450 Issue 3 Pg. 210-6 (May 07 1999) ISSN: 0014-5793 [Print] England
• PMID: 10359076 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Isoenzymes
• Ki-67 Antigen
• Type C Phospholipases
• PLCB3 protein, human
• Phospholipase C beta
• Plcb3 protein, mouse
• Plcb3 protein, rat

Topics

• Animals
• Cell Division
• Female
• Humans
• Isoenzymes (biosynthesis, genetics)
• Ki-67 Antigen (metabolism)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Neuroendocrine Tumors (enzymology, etiology)
• Phenotype
• Phospholipase C beta
• Rats
• Transfection
• Tumor Cells, Cultured
• Type C Phospholipases (biosynthesis, genetics)