[The effect of natural mineral bicarbonate sodium-calcium water «Tib-1» on the antioxidant status of rats with adjuvant rheumatoid arthritis].

Abstract

The combination of traditional basic pharmacotherapy for rheumatoid arthritis (RA) and physiotherapeutic methods can reduce the activity of the disease and accelerate the onset of remission, and therefore the development of new non-drug methods for the treatment of RA is relevant.

PURPOSE OF THE STUDY:

Study of the effect of natural mineral water « Tib-1» on the lipid peroxidation system in an experiment with a model adjuvant-induced RA in rats.

MATERIAL AND METHODS:

The object of the study were Wistar rats, divided into three groups: negative control (solvents), positive control (model of adjuvant-induced RA by subcutaneous injection of complete Freund's adjuvant) and experimental (correction of RA with mineral water « Tib-1», diluted in a ratio of 1:3 during the first 2 weeks from the moment the model was formed in the ad libitum mode). On the 3rd and 7th weeks in the blood of the animals were determined: the total number of leukocytes, the content of hydroperoxides according to Gavrilov, the level of malondialdehyde (MDA), catalase activity. Pathological changes in the hip and knee joints were recorded using radiography.

RESULTS:

The inflammatory process in the positive control group by the 3rd week was characterized by an increase in the number of leukocytes by 66% (p<0.01) and was accompanied by an increase in MDA by 60% (p<0.001). By the 7th week, despite a relative increase in catalase activity (16%), the MDA level continued to be elevated compared to the negative control by 67% (p<0.001). Against the background of exposure to mineral water, inflammation decreased (the number of leukocytes in the "model/experiment" groups turned out to be reduced by 41%; p<0.01) and an increase in compensatory-adaptive reactions in the form of catalase activation was noted (by 8%; p<0.01), which was accompanied by a persistent (weeks 3 and 7) decrease in MDA output (by 20%; p<0.01). Using the method of radiation diagnostics, positive changes in the articular apparatus of experimental animals were revealed, consisting in the relief of signs of subchondral sclerosis of the bone heads, which were noted for animals of the model group.

CONCLUSION:

The use of natural mineral water «Tib-1» helps to reduce the acute inflammatory response during the formation of adjuvant-induced RA in Wistar rats, initiates the normalization of the balance of pro- and antioxidant processes in the body, and minimizes the intensity of degenerative-inflammatory joint lesions.

• Authors: S V Skupnevskii, F E Batagova, E G Pukhaeva, A K Badtiev, F K Rurua, Zh G Farnieva
• Journal: Voprosy kurortologii, fizioterapii, i lechebnoi fizicheskoi kultury (Vopr Kurortol Fizioter Lech Fiz Kult) Vol. 99 Issue 3 Pg. 41-49 ( 2022) ISSN: 0042-8787 [Print] Russia (Federation)
• Vernacular Title: Vliyanie prirodnoi mineral'noi gidrokarbonatnoi natrievo-kal'tsievoi vody «Tib-1» na antioksidantnyi status krys s ad"yuvantnym revmatoidnym artritom.
• PMID: 35700375 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Bicarbonates
• Mineral Waters
• Minerals
• Sodium
• Catalase
• Calcium

Topics

• Animals
• Antioxidants (pharmacology, therapeutic use)
• Arthritis, Experimental (drug therapy)
• Arthritis, Rheumatoid (drug therapy)
• Bicarbonates (adverse effects)
• Calcium (therapeutic use)
• Catalase (therapeutic use)
• Inflammation
• Mineral Waters (therapeutic use)
• Minerals (therapeutic use)
• Rats
• Rats, Wistar
• Sodium (adverse effects)