Abstract |
The sensitivity to photosensitization mediated by the hematoporphyrin photosensitizer PSD-007 of acute promyelocytic leukemic cell line (HL-60) was compared with normal human hemopoietic progenitor cells. The results showed that the leukemic cells were more sensitive. After being treated with 10 micrograms.ml-1 PSD-007 followed by 2J.cm-2 copper vapor laser light irradiation, the clonogenic leukemic (HL-60) cells were reduced 98%, but the survival rate of human granulocyte-macrophage colony-forming units (CFU-GM) was 40 +/- 8%. Mixing of normal human marrow cells with leukemic (HL-60) cells (ratio 100:1) did not interfere with elimination of tumor cells. The ultrastructure changes of HL-60 cells treated by laser photoradiation was observed under the trans-electronic microscope. The mitochondria, endoplasmic reticulum and cell membrane were involved. This means that the cell biomembrane is the main target to be attacked. It is considered that PSD-007-laser photoradiation therapy is efficient for killing leukemic cells.
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Authors | X Liang, Z Qi, P Cao, J Xiao, G Tang, X Li, J Lai |
Journal | Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University
(Hunan Yi Ke Da Xue Xue Bao)
Vol. 22
Issue 3
Pg. 199-202
( 1997)
ISSN: 1000-5625 [Print] China |
PMID | 9868111
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Photosensitizing Agents
- Hematoporphyrin Derivative
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Topics |
- Adult
- Bone Marrow Purging
- Bone Marrow Transplantation
- HL-60 Cells
(drug effects, ultrastructure)
- Hematopoietic Stem Cells
(drug effects)
- Hematoporphyrin Derivative
(pharmacology)
- Hematoporphyrin Photoradiation
- Humans
- Photosensitizing Agents
(pharmacology)
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