Abstract |
Nalidixic acid (NA) has been used for urinary tract infections and has been reported to be photocarcinogenic. We examined the mechanism of damage to 32P-labeled DNA fragments obtained from the human c-Ha-ras-1 proto-oncogene and the p53 tumor suppressor gene exposed to 365-nm UVA light in the presence of NA. NA plus UVA light caused damage to the double-stranded DNA fragment at consecutive guanine residues, whereas damage to the single-stranded DNA fragment was caused at single guanines and thymines. The formation of 8-oxo-7, 8-dihydro-2'-deoxyguanosine in native DNA exceeded that in denatured DNA at high NA concentrations. The ESR spin destruction method suggested that DNA damage was caused through electron transfer from guanine residues to photoexcited NA. On the basis of these findings, it is concluded that NA can cause skin cancer through DNA damage mediated by its photoactivation.
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Authors | Y Hiraku, H Ito, S Kawanishi |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 251
Issue 2
Pg. 466-70
(Oct 20 1998)
ISSN: 0006-291X [Print] United States |
PMID | 9792797
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 1998 Academic Press. |
Chemical References |
- MAS1 protein, human
- Oligodeoxyribonucleotides
- Proto-Oncogene Mas
- Poly G
- Nalidixic Acid
- DNA
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Topics |
- Base Sequence
- DNA
(chemistry, drug effects, radiation effects)
- DNA Damage
- Dose-Response Relationship, Drug
- Dose-Response Relationship, Radiation
- Electron Spin Resonance Spectroscopy
- Genes, p53
- Humans
- Hydroxylation
- Kinetics
- Molecular Sequence Data
- Nalidixic Acid
(pharmacology)
- Nucleic Acid Denaturation
- Oligodeoxyribonucleotides
(chemistry, radiation effects)
- Poly G
- Proto-Oncogene Mas
- Ultraviolet Rays
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