Short- and long-term efficacy of single-dose subunit vaccines against Yersinia pestis in mice.

Abstract

A single, subcutaneous, 30-microg dose of either a combination of the Yersinia pestis proteins F1+V or a F1-V fusion protein adsorbed to the adjuvant aluminum hydroxide, protected Hsd:ND4 mice for one year against pneumonic plague. The recombinant F1+V vaccine provided significant protection as early as day 14 postimmunization. The current Plague Vaccine USP in a single 0.2-ml dose did not provide significant protection in this mouse model. Antibody titers to F1 and V peaked at approximately 5-12 weeks postimmunization and were still detectable one year later. These F1 and V subunit vaccines may offer effective long-term immunity with a reduced dosage schedule when compared with the presently licensed, formalin-killed, whole-cell vaccine.

• Authors: G W Anderson Jr, D G Heath, C R Bolt, S L Welkos, A M Friedlander
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 58 Issue 6 Pg. 793-9 (Jun 1998) ISSN: 0002-9637 [Print] United States
• PMID: 9660466 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Plague Vaccine
• Recombinant Fusion Proteins
• Recombinant Proteins
• Vaccines, Synthetic

Topics

• Animals
• Antibodies, Bacterial (biosynthesis)
• Antigens, Bacterial (immunology)
• Dose-Response Relationship, Immunologic
• Enzyme-Linked Immunosorbent Assay
• Female
• Mice
• Plague (prevention & control)
• Plague Vaccine (administration & dosage, immunology, standards)
• Recombinant Fusion Proteins (immunology)
• Recombinant Proteins (immunology)
• Vaccines, Synthetic (administration & dosage, immunology, standards)
• Yersinia pestis (immunology)