Expression of a 260 kDa neuroblastoma surface antigen, the target of cytotoxic natural human IgM: correlation to MYCN amplification and effects of retinoic acid.

Abstract

Human neuroblastoma cells contain a 260 kDa surface-associated antigen (NB-p260) that is recognised by natural cytotoxic IgM antibodies. In this study we demonstrate that NB-p260 is expressed in vivo in a neuroblastoma tumour specimen but not in normal human tissues of neuronal origin. Since MYCN amplification is a clinical marker of neuroblastoma disease progression, we analysed the expression of NB-p260 in human neuroblastoma cell lines with different MYCN amplification status. However, both amplified and non-amplified neuroblastoma cell lines exhibited comparable NB-p260 expression. Treatment of neuroblastoma cells with the differentiation-inducing agent retinoic acid (RA) also had no effect on the expression of NB-p260. Collectively, the data suggest that expression of NB-p260 on human neuroblastoma cells is independent of malignancy and differentiation status of neuroblastoma.

• Authors: K David, A Ehrhardt, M W Ollert, R Erttmann, R Bredehorst, C W Vogel
• Journal: European journal of cancer (Oxford, England : 1990) (Eur J Cancer) Vol. 33 Issue 12 Pg. 1937-41 (Oct 1997) ISSN: 0959-8049 [Print] England
• PMID: 9516828 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Antigens, Surface
• Immunoglobulin M
• Tretinoin

Topics

• Antigens, Neoplasm (immunology, metabolism)
• Antigens, Surface (immunology, metabolism)
• Cell Transformation, Neoplastic
• Gene Amplification
• Gene Expression
• Genes, myc (genetics)
• Humans
• Immunoblotting
• Immunoglobulin M (immunology)
• Immunohistochemistry
• Neuroblastoma (genetics, immunology, metabolism, pathology)
• Tretinoin (pharmacology)
• Tumor Cells, Cultured (drug effects)