The study was performed on rats divided into 9 groups. Groups 1-3 served as controls. In groups 4 and 5 rat livers were subjected to 90-min
ischemia followed by 12- or 24-hour reperfusion. In groups 6 and 7 rats were injected with intraperitoneal
chlorfenvinphos (2 mg/kg b.w.) and sacrificed after 12 or 24 hours. In groups 8 and 9 rat livers were subjected to 90-min
ischemia, 12- or 24-hour reperfusion and then rats were injected with
chlorfenvinphos (2 mg/kg b.w.). Liver sections were evaluated morphologically, histochemically (SDH, LDH, G6Pase,
glycogen,
Mg2+ ATPase and AcP). The microsomal fraction of the liver was evaluated for
cytochrome P450 content and
NADPH-cytochrome P-450 reductase activity. It has been found that liver
ischemia and reperfusion result in extensive
necrosis, enzymatic disturbances, particularly in acinar zone 3.
Ischemia as well as reperfusion decrease the
cytochrome P450 content of hepatocytic microsomes and the activity of
NADPH-cytochrome P-450 reductase.
Intraperitoneal injection of
chlorfenvinphos during
ischemia and reperfusion dramatically intensifies damage to the liver, although
chlorfenvinphos alone produces only mild nonspecific effects on the morphological and enzymatic structure of the liver.