Abstract | BACKGROUND: METHODS: Human pheochromocytoma and PC12 rat pheochromocytoma cells were grown in medium supplemented with suramin at concentrations of 1-1,000 micrograms/ml (1.43-1.43 mM) for up to five generations. Suramin did not induce neuronal differentiation, but inhibited growth of cultured human pheochromocytoma cells with IC50 (inhibitory concentration at which a 50% reduction of proliferation is observed) of 50-250 micrograms/ml. Also, suramin inhibited proliferation of PC12 cells with IC50 of 228 micrograms/ml after 5 days and 161 micrograms/ml at 10 days of treatment. Colony formation assays demonstrated these effects to be cytotoxic rather than cytostatic. Thus when reproductive integrity of PC12 cells was taken into account, IC50 was calculated with 118 micrograms/ml and 129 micrograms/ml, respectively. In vivo experiments were performed with subcutaneously xenotransplanted PC12 cells (BALB/c NCR-NU mice). Suramin did not alter tumorigenicity and did not inhibit local tumor growth. RESULTS: CONCLUSIONS:
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Authors | A Zielke, M G Wong, A E Siperstein, O H Clark, M Rothmund, Q Y Duh |
Journal | Journal of surgical oncology
(J Surg Oncol)
Vol. 66
Issue 1
Pg. 11-8
(Sep 1997)
ISSN: 0022-4790 [Print] United States |
PMID | 9290687
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Antineoplastic Agents
- Suramin
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Topics |
- Adrenal Gland Neoplasms
(pathology)
- Animals
- Antineoplastic Agents
(pharmacology)
- Cell Division
(drug effects)
- Cell Survival
(drug effects)
- Drug Screening Assays, Antitumor
- Female
- Humans
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Neoplasm Transplantation
- PC12 Cells
(drug effects)
- Pheochromocytoma
(pathology)
- Rats
- Suramin
(pharmacology)
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