Human immunodeficiency virus strains differ in their ability to infect CD4+ cells expressing the rat homolog of CXCR-4 (fusin).

Abstract

A clade B strain of human immunodeficiency virus type 1 (HIV-1(LAI)) could infect CD4+ cells expressing human CXCR-4 (fusin) or its rat homolog with similar efficacy. By contrast, cells expressing rat CXCR-4 were not permissive to HIV-1(NDK) (clade D), HIV-2(ROD), or HIV-1(LAI) with chimeric envelope protein gp120 bearing the V3 domain from HIV-1(NDK). The reciprocal chimeric gp120 (HIV-1(NDK) with V3 from HIV-1(LAI)) could mediate infection of cells expressing either human or rat CXCR-4. Genetically divergent HIV strains have different requirements for interaction with the CXCR-4 coreceptor, and the gp120 V3 domain seems to be involved in this interaction.

• Authors: O Pleskoff, N Sol, B Labrosse, M Alizon
• Journal: Journal of virology (J Virol) Vol. 71 Issue 4 Pg. 3259-62 (Apr 1997) ISSN: 0022-538X [Print] United States
• PMID: 9060691 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HIV Core Protein p24
• HIV Envelope Protein gp120
• HIV envelope protein gp120 (305-321)
• Membrane Proteins
• Peptide Fragments
• Receptors, CXCR4
• Receptors, HIV
• Recombinant Fusion Proteins

Topics

• Animals
• CD4-Positive T-Lymphocytes (metabolism, virology)
• HIV Core Protein p24 (analysis)
• HIV Envelope Protein gp120 (genetics, immunology)
• HIV-1 (classification, genetics, physiology)
• HIV-2 (classification, physiology)
• HeLa Cells
• Humans
• Membrane Proteins (biosynthesis, genetics, physiology)
• Peptide Fragments (genetics, immunology)
• Rats
• Receptors, CXCR4
• Receptors, HIV (biosynthesis, genetics, physiology)
• Recombinant Fusion Proteins (genetics)
• Species Specificity
• Tumor Cells, Cultured