Complementation of divergent mga genes in group A Streptococcus.

Abstract

The gene (mga4) encoding the positive regulatory protein, Mga4, was cloned and sequenced from an M type 4 strain (AP4) of Streptococcus pyogenes. The deduced amino acid (aa) sequence of this "divergent Mga' shows 88% identity to the prototype Mga1 in its N-terminal half, which contains all three of the predicted helix-turn-helix domains. However, one of the predicted receiver domains of Mga1, which is at its C terminus, is not conserved in the Mga4 aa sequence. Nevertheless, a mutation in mga1 was found to be complemented for activation of the gene encoding M protein (emm) by mga4 in trans. This suggests that the aa residues of the C-terminal predicted receiver domain are not critical for activation of emm transcription.

• Authors: G Andersson, K McIver, L O Hedén, J R Scott
• Journal: Gene (Gene) Vol. 175 Issue 1-2 Pg. 77-81 (Oct 10 1996) ISSN: 0378-1119 [Print] Netherlands
• PMID: 8917079 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Bacterial Proteins
• Mga4 protein, Streptococcus pyogenes
• Trans-Activators
• mry protein, Streptococcus pyogenes

Topics

• Amino Acid Sequence
• Bacterial Proteins (genetics, metabolism)
• Base Sequence
• Genes, Bacterial (genetics)
• Genes, Regulator (genetics)
• Molecular Sequence Data
• Streptococcus pyogenes (genetics)
• Trans-Activators (genetics, metabolism)
• Transcription, Genetic