Abstract |
To investigate the extra susceptibility of diabetics to some nephrotoxic agents, adult normal and diabetic Chinese hamsters (6-7 animals in each group) were injected subcutaneously with different doses of cadmium-metallothionein (Cd-MT) equivalent to 0.0, 0.1 or 0.25 mg Cd/kg body weight and the first 24 hr urinary outputs were collected. Several days prior to exposure to the Cd-MT the diabetic hamsters were hyperglycaemic, and plasma insulin levels and body weights were elevated in some of the diabetics. The higher dose of Cd-MT caused significant spillage of N-acetyl-beta- glucosaminidase (U-NAG) activity and protein into the urine of both normal and diabetic animals. The higher dose of Cd-MT was more toxic to the diabetic kidneys because U-NAG levels were higher in the diabetics (2.5-fold higher than normal). U-Cd levels were proportional to the injected Cd-MT dose. U-Zn levels were not consistently affected by the injected Cd-MT although it had contained small amounts of Zn. Therefore, genetic diabetes in the Chinese hamster appears to increase susceptibility to acute cadmium-MT nephrotoxicity. The mechanisms underlying this need to be further investigated.
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Authors | T Jin, B J Frankel |
Journal | Pharmacology & toxicology
(Pharmacol Toxicol)
Vol. 79
Issue 3
Pg. 105-8
(Sep 1996)
ISSN: 0901-9928 [Print] Denmark |
PMID | 8884866
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Blood Glucose
- Insulin
- Cadmium
- Metallothionein
- Acetylglucosaminidase
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Topics |
- Acetylglucosaminidase
(urine)
- Analysis of Variance
- Animals
- Blood Glucose
(analysis)
- Body Weight
(drug effects, physiology)
- Cadmium
(administration & dosage, toxicity, urine)
- Cricetinae
- Cricetulus
- Diabetes Mellitus
(genetics, physiopathology, urine)
- Dose-Response Relationship, Drug
- Injections, Subcutaneous
- Insulin
(blood)
- Kidney
(drug effects, pathology)
- Metallothionein
(administration & dosage, toxicity)
- Proteinuria
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