In this study, we used immunohistochemical and biochemical analysis to show that
gp200-MR6, a 200 kDa molecule that is functionally associated with the human
interleukin 4 (IL-4) receptor complex, is expressed at high levels on normal breast epithelial tissues, at lower levels on in situ
carcinomas, and that the expression is lost in the invasive
carcinoma of the breast. Furthermore, a preliminary study showed that benign epithelial
hyperplasia of the breast expresses the
gp200-MR6 heterogeneously. Two populations of cells have been observed: MR6 positive and MR6 negative. Interestingly, MR6-positive cells were observed to have different morphology from those that were MR6 negative; the nuclei of the former were larger and rounded in shape, whereas the nuclei of the latter were relatively small and oval in shape. In
sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting,
monoclonal antibody MR6 detects the same molecular weight molecule in both normal and transformed tissue, indicating that the molecule is not a product of a truncated gene. The intensity of the
gp200-MR6 bands correlates with the immunohistochemical data, indicating that the molecule is expressed at high levels in normal tissue and at lower levels in malignant tissue. These results suggest that analysis of
gp200-MR6 expression may be useful in tumour grading and prognostic evaluation in
breast cancer. Moreover, the molecule may be involved early in the process of
tumorigenesis of the breast, in which a loss or a down-regulation of
gp200-MR6 could contribute towards tumour development and progression via an effect on cell growth and differentiation.