Abstract | BACKGROUND: Nasal challenge with platelet activating factor (PAF) is able to induce local neutrophilia, with a different degree of responsiveness in atopic subjects and in nonatopic subjects. We investigated whether nasal accumulation of neutrophils induced by PAF is accompanied by the release of neutrophil-derived mediators. METHODS: RESULTS: PAF caused neutrophilia, which appeared after 30 minutes in atopic subjects and after 3 hours in nonatopic subjects. Furthermore, when compared with saline insufflation, PAF caused a significant release of MPO in the nasal lavage fluids collected 30 minutes, 3 hours, and 24 hours after challenge in atopic subjects and 3 hours after challenge in nonatopic subjects, with higher values in the former than in the latter. Neutrophil counts correlated with MPO levels in the nasal lavages collected after PAF challenge. A lower degree of neutrophilia was found 3 hours after stimulation with lyso-PAF in both groups of subjects, with a marginal release of MPO in atopic subjects only. No significant increase of immunoreactive leukotriene B4 levels in nasal lavages was found after challenge with either PAF or lyso-PAF. CONCLUSION: These results indicate that PAF-induced neutrophilia in the nose is accompanied by the release of MPO, which appears earlier and is more marked in atopic subjects than in nonatopic subjects.
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Authors | A Miadonna, N Milazzo, M Lorini, A Sala, A Tedeschi |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 97
Issue 4
Pg. 947-54
(Apr 1996)
ISSN: 0091-6749 [Print] United States |
PMID | 8655890
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Platelet Activating Factor
- Leukotriene B4
- Sodium Chloride
- Peroxidase
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Topics |
- Administration, Intranasal
- Adult
- Female
- Humans
- Hypersensitivity, Immediate
(enzymology, immunology, pathology)
- Leukocyte Count
- Leukocytosis
(chemically induced, enzymology, immunology)
- Leukotriene B4
(metabolism)
- Male
- Nasal Lavage Fluid
(chemistry, cytology, immunology)
- Neutrophils
(drug effects, enzymology, immunology)
- Peroxidase
(drug effects, metabolism)
- Platelet Activating Factor
(administration & dosage, pharmacology)
- Rhinitis, Allergic, Seasonal
(enzymology, immunology, pathology)
- Sodium Chloride
(pharmacology)
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