Nonenzymic glycation of apolipoprotein B in patients with insulin- and noninsulin-dependent diabetes mellitus.

Abstract

OBJECTIVE:

To evaluate the level of nonenzymatic glycation of apolipoprotein B in patients with insulin and noninsulin dependent diabetes mellitus.

METHODS:

Using a method based on a combination of affinity chromatography and immunonephelometry, we measured the concentration of glycated apolipoprotein B (apo B) in serum of 140 diabetic patients, 43 insulin-dependent (IDDM), and 97 noninsulin-dependent (NIDDM), and 45 nondiabetic control subjects.

RESULTS:

Although total apo B concentration in serum was significantly increased only in NIDDM patients, both groups of diabetics showed higher percentages of glycated apo B (IDDM, 4.84 +/- 0.8%; NIDDM, 5.61 +/- 1.1%) than did control subjects (4.28 +/- 1.0%), the greatest percentage (5.80%) being found in patients with diabetic nephropathy. No significant correlations were found between glycated apo B and the traditional parameters of glycemic control, such as glycated hemoglobin and fructosamines, and direct influence by sudden plasma glucose fluctuations on apo B glycation was not shown either, perhaps for a low inherent glycability of this apolipoprotein.

CONCLUSIONS:

It is unclear if these low proportions of glycated apo B in vivo may significantly affect lipoprotein metabolism assuming a pathophysiological role in atherogenesis.

• Authors: M Panteghini, A Cimino, F Pagani, A Girelli
• Journal: Clinical biochemistry (Clin Biochem) Vol. 28 Issue 6 Pg. 587-92 (Dec 1995) ISSN: 0009-9120 [Print] United States
• PMID: 8595706 (Publication Type: Journal Article)

Chemical References

• Apolipoproteins B
• Blood Glucose
• Glycated Hemoglobin A
• Hexosamines

Topics

• Adolescent
• Adult
• Aged
• Apolipoproteins B (blood, metabolism)
• Blood Glucose (analysis)
• Blood Glucose Self-Monitoring
• Chromatography, Affinity (methods)
• Diabetes Mellitus, Type 1 (blood, complications)
• Diabetes Mellitus, Type 2 (blood, complications)
• Diabetic Angiopathies (blood, metabolism)
• Female
• Glycated Hemoglobin (analysis)
• Glycosylation
• Hexosamines (blood)
• Humans
• Male
• Middle Aged
• Nephelometry and Turbidimetry