Inhibition of CMP-sialic acid transport in human liver and colorectal cancer cell lines by a sialic acid nucleoside conjugate (KI-8110).

Abstract

The sialic acid nucleoside conjugate KI-8110 has been shown to inhibit the formation of hepatic metastases from human colorectal cancer cell lines in a nude mouse intrasplenic injection model. The compound does not inhibit sialyltransferases from either human colorectal tumor cells or human liver. Transport of CMP-sialic acid into endoplasmic reticulum and Golgi vesicles is inhibited and can account for the reduction in surface sialic acid found on treated cell lines. Only a 50% inhibition of CMP-sialic acid transport could be achieved suggesting the presence of more than one transport protein with differing specificities.

• Authors: B E Harvey, P Thomas
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 190 Issue 2 Pg. 571-5 (Jan 29 1993) ISSN: 0006-291X [Print] United States
• PMID: 8427599 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Glycosides
• Cytidine Monophosphate N-Acetylneuraminic Acid
• KI 8110
• Sialyltransferases
• Cytidine Monophosphate
• Uridine
• beta-D-Galactoside alpha 2-6-Sialyltransferase

Topics

• Binding, Competitive
• Biological Transport (drug effects)
• Colorectal Neoplasms (metabolism)
• Cytidine Monophosphate (pharmacology)
• Cytidine Monophosphate N-Acetylneuraminic Acid (metabolism)
• Glycosides (pharmacology)
• Humans
• Liver (drug effects, metabolism)
• Microsomes (enzymology)
• Microsomes, Liver (enzymology)
• Sialyltransferases (antagonists & inhibitors, metabolism)
• Tumor Cells, Cultured
• Uridine (analogs & derivatives, pharmacology)
• beta-D-Galactoside alpha 2-6-Sialyltransferase