Expression of viral T-antigen in pathological tissues from transgenic mice carrying JC-SV40 chimeric DNAs.

Abstract

Immunostaining methods were used to detect viral T-antigen and the cellular protein p53 in pathological tissues obtained from transgenic mice carrying JC-SV40 hybrid viral DNAs. A transgenic mouse carrying the SV40 regulatory region and JC virus (JCV) T-antigen-coding sequences exhibited an SV40-characteristic choroid plexus papilloma that expressed JCV T-antigen and p53. JCV-associated pathology was observed in two other mice in which the JCV regulatory signals directed SV40 T-antigen-induced adrenal neuroblastomas and brain neoplastic cells. However, these mice also exhibited an SV40-characteristic osteosarcoma and abdominal lymphoma that contained SV40 T-antigen and p53-positive cells. Contrasting thymic pathology was observed in the two types of mice where the SV40 regulatory region directed a JCV T-antigen-induced thymoma in one mouse, and the JCV regulatory region directed SV40 T-antigen-induced thymic hypoplasia in two other mice.

• Authors: H G Ressetar, O Prakash, R J Frisque, H D Webster, R N Re, G L Stoner
• Journal: Molecular and chemical neuropathology (Mol Chem Neuropathol) Vol. 20 Issue 1 Pg. 59-79 (Sep 1993) ISSN: 1044-7393 [Print] United States
• PMID: 8251033 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Polyomavirus Transforming
• DNA, Viral

Topics

• Adrenal Gland Neoplasms (pathology)
• Animals
• Antigens, Polyomavirus Transforming (genetics, immunology)
• Brain Neoplasms (pathology)
• Chimera
• Choroid Plexus Neoplasms (pathology)
• DNA, Viral (genetics, isolation & purification)
• Female
• Genome, Viral
• Immunohistochemistry
• Lymphoma (pathology)
• Mice
• Mice, Inbred C57BL
• Mice, Inbred CBA
• Mice, Transgenic
• Neuroblastoma (pathology)
• Osteosarcoma (pathology)
• Papilloma (pathology)
• Pregnancy
• Skull Neoplasms (pathology)
• Thymoma (pathology)
• Thymus Neoplasms (pathology)