Abstract |
Activated c-N-ras alleles have been detected in human lymphoma specimens. The aim of the present study was to determine the frequency of c-N-ras mutational activation in canine malignant lymphoma. DNA was isolated from 28 canine malignant lymphoma specimens collected from 28 separate dogs and examined for c-N-ras mutations by polymerase chain reaction amplification and direct sequencing. The tumors were naturally occurring and derived from 20 dogs with known exposures to the phenoxy herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) and from 8 dogs with no known exposure to the herbicide. An oncogenically activating mutation was found in 1 dog without known 2,4-D exposure. The mutation was a 13th codon, second position transition that would result in a glycine-to- aspartate amino acid substitution. The results of this study demonstrate that, similar to the human, c-N-ras mutations are uncommon in dogs with malignant lymphoma and that there is no association between 2,4-D exposure and activation of c-N-ras in the dog.
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Authors | M D Edwards, K A Pazzi, P H Gumerlock, B R Madewell |
Journal | Toxicologic pathology
(Toxicol Pathol)
Vol. 21
Issue 3
Pg. 288-91
( 1993)
ISSN: 0192-6233 [Print] United States |
PMID | 8248717
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Carcinogens
- DNA, Neoplasm
- 2,4-Dichlorophenoxyacetic Acid
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Topics |
- 2,4-Dichlorophenoxyacetic Acid
- Animals
- Base Sequence
- Carcinogens
- DNA, Neoplasm
(biosynthesis)
- Dog Diseases
(genetics)
- Dogs
- Exons
- Genes, ras
- Histocytochemistry
- Lymphoma
(chemically induced, genetics, veterinary)
- Mutation
- Polymerase Chain Reaction
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