Localization of the gene (RSN) coding for restin, a marker for Reed-Sternberg cells in Hodgkin's disease, to human chromosome band 12q24.3 and YAC cloning of the locus.

Abstract

A novel 160-kDa intermediate filament associated protein, named restin (Reed-Sternberg intermediate filament associated protein), is specifically expressed in the malignant cells of Hodgkin's disease and anaplastic large cell lymphoma (Ki-1 lymphoma). The combination of chromosomal R-banding and fluorescence in situ hybridization (FISH) with the use of two fluorescent dyes, fluorescein isothiocyanate and propidium iodide, allowed simultaneous detection of the hybridized DNA sequence and chromosomal R-banding. By this technique, the gene coding for restin (RSN) was assigned to chromosome region 12q24.31-->q24.33, while localization of the alpha-2-macroglobulin receptor (A2MR) was refined to 12q13.1-->q13.3. To further analyze the restin gene, a 500-kb YAC clone containing the gene was isolated and analyzed. A restriction map of this area is presented.

• Authors: C Hilliker, J Delabie, F Speleman, G Bilbe, J Bruggen, F Van Leuven, H Van den Berghe
• Journal: Cytogenetics and cell genetics (Cytogenet Cell Genet) Vol. 65 Issue 3 Pg. 172-6 ( 1994) ISSN: 0301-0171 [Print] Switzerland
• PMID: 8222754 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Complementary
• Intermediate Filament Proteins
• Microtubule-Associated Proteins
• Neoplasm Proteins
• cytoplasmic linker protein 170

Topics

• Chromosome Banding
• Chromosome Mapping
• Chromosomes, Artificial, Yeast
• Chromosomes, Human, Pair 12
• Cloning, Molecular
• DNA, Complementary (genetics)
• Humans
• In Situ Hybridization, Fluorescence
• Intermediate Filament Proteins (genetics)
• Microtubule-Associated Proteins
• Neoplasm Proteins (genetics)
• Reed-Sternberg Cells (ultrastructure)
• Restriction Mapping