Use of capillary electrophoresis-electrospray ionization mass spectrometry in the analysis of synthetic peptides.

Abstract

We have constructed a capillary electrophoresis (CE) system with UV detection and have successfully interfaced it to an electrospray ionization mass spectrometry (ES-MS) system. A synthesized fragment of heregulin-beta (212-226) was thought to be a single component by re-injection into an HPLC system, but results from CE-UV-ES-MS indicated that a dehydration product was present in the desired peptide sample. A synthetic heregulin-alpha (177-241) was isolated by preparative HPLC, but re-injection on an analytical system indicated a tailing peak. CE-UV-ES-MS indicated a mixture whose two major components were of the same nominal molecular mass (within experimental error), suggesting the presence of an isomer or a deamidation product. The results show that CE-UV-ES-MS can be used as an orthogonal analytical technique to solve practical problems encountered in peptide synthesis laboratories.

• Authors: K J Rosnack, J G Stroh, D H Singleton, B C Guarino, G C Andrews
• Journal: Journal of chromatography. A (J Chromatogr A) Vol. 675 Issue 1-2 Pg. 219-25 (Jul 22 1994) ISSN: 0021-9673 [Print] Netherlands
• PMID: 8081459 (Publication Type: Journal Article)

Chemical References

• Carrier Proteins
• Glycoproteins
• Ions
• Neuregulin-1
• Neuregulins
• Peptide Fragments
• heregulin beta (212-226)

Topics

• Amino Acid Sequence
• Carrier Proteins (analysis)
• Electrons
• Electrophoresis (methods)
• Glycoproteins (analysis)
• Ions
• Mass Spectrometry (methods)
• Molecular Sequence Data
• Neuregulin-1
• Neuregulins
• Peptide Fragments (analysis)
• Spectrophotometry, Ultraviolet