Abstract | BACKGROUND AND DESIGN: Immunofluorescence detection of stippled IgA in dermal papillae has been considered the gold standard in the diagnosis of dermatitis herpetiformis (DH). We have developed an immunohistochemical technique using the avidin- biotin- peroxidase complex that is equally effective as direct immunofluorescence in detecting IgA. We retrospectively studied 43 paraffin-embedded biopsy specimens obtained from patients with DH and a variety of other diseases for the presence of IgA along the basement membrane zone. RESULTS: CONCLUSION:
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Authors | A L Zaenglein, L Hafer, K F Helm |
Journal | Archives of dermatology
(Arch Dermatol)
Vol. 131
Issue 5
Pg. 571-3
(May 1995)
ISSN: 0003-987X [Print] United States |
PMID | 7741544
(Publication Type: Journal Article)
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Chemical References |
- Immunoglobulin A
- Avidin
- Biotin
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Topics |
- Avidin
- Basement Membrane
(immunology, pathology)
- Biopsy
- Biotin
- Dermatitis
(diagnosis, immunology, pathology)
- Dermatitis Herpetiformis
(diagnosis, immunology, pathology)
- Fluorescent Antibody Technique
- Humans
- Immunoenzyme Techniques
- Immunoglobulin A
(analysis)
- Lupus Erythematosus, Discoid
(diagnosis, immunology, pathology)
- Paraffin Embedding
- Pemphigoid, Bullous
(diagnosis, immunology, pathology)
- Pemphigus
(diagnosis, immunology, pathology)
- Retrospective Studies
- Skin Diseases, Vesiculobullous
(diagnosis, immunology, pathology)
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