Use of a novel system for defining a gene imprinting region.

Abstract

Gene imprinting involves the expression of a single allele, depending on its parental origin. In this report, we describe the use of a novel system, implementing human tissues exclusively endowed with either maternally or paternally inherited chromosomes, to better define a known gene imprinting region. Specific RNA transcripts for Placental Ribonuclease Inhibitor (PRI) and Cathepsin D were analysed by northern blotting for expression in complete hydatidiform mole, mature teratoma, and normal placenta. These genes are in close proximity to the reciprocally imprinted H19 and IGF-2 genes found on chromosome 11p15.5. Since all the tissues studied expressed Cathepsin D and PRI, these are not, by definition, imprinted, but as yet we cannot define the borders of the imprinting area on chromosome 11p15.5.

• Authors: J Rachmilewitz, B Gonik, R Goshen, I Ariel, T Schneider, N de Groot, A Hochberg
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 196 Issue 2 Pg. 659-64 (Oct 29 1993) ISSN: 0006-291X [Print] United States
• PMID: 7694575 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• RNA, Neoplasm
• RNA
• Cathepsin D

Topics

• Alleles
• Blotting, Northern
• Cathepsin D (biosynthesis, genetics)
• Chromosomes, Human, Pair 11
• Female
• Gene Expression
• Humans
• Hydatidiform Mole (metabolism)
• Male
• Placenta (metabolism)
• Pregnancy
• Pregnancy Trimester, First
• RNA (isolation & purification, metabolism)
• RNA, Neoplasm (isolation & purification, metabolism)
• Teratoma (metabolism)
• Uterine Neoplasms (metabolism)